Effect of Nutritional INterventions on Autophagy in healthy adults (The NINA Study)

This is a dataset of 63 participants that measures the effect of a four week modification of dietary macronutrient intake on autophagic flux (i.e. a cell mechanism that cleanses the cells) in fasting healthy individuals. This study is a single-site, single blinded, randomised crossover trial, where participants will be randomised to one of two study diets for four weeks before crossing over to the other diet, with a four week washout period between diets. The primary end point is the change in autophagic flux between an average macronutrient diet compared to altered macronutrient diet (randomly assigned crossover design). Assessed by measuring the amount of the autophagic cargo LC3 (with or without the lysosome inhibitor chloroquine) in peripheral blood mononuclear cells by ELISA.