A method to isolate and purify human bone marrow stromal stem cells

Stan Gronthos; Andrew C W Zannettino

doi:10.1007/978-1-60327-169-1_3

A method to isolate and purify human bone marrow stromal stem cells

Stan Gronthos, Andrew C W Zannettino

Blood Cancer Program

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

53 Citations (Scopus)

Abstract

The STRO-1 antibody can be used as a single reagent to isolate human bone marrow stromal stem cells (BMSSC), owing to its restricted specificity to a cell surface molecule expressed by clonogenic BMSSC, with little or no reactivity to hematopoietic stem/progenitor populations or mature stromal elements. The present protocol uses a combination of two different immunoselection methodologies in an attempt to generate highly purified preparations of BMSSC. This process involves the initial isolation of a minor subpopulation of bone marrow mononuclear cells (approx 10%) expressing the STRO-1 antigen, by means of magnetic activated cell sorting. Dual-color fluorescence activated cell sorting is then used as a secondary step to further purify the rare STRO-1 ^bright expressing fraction that contains all of the colony-forming BMSSC, based on their co-expression of a secondary cell surface marker, CD106 (VCAM-1).

Original language	English
Title of host publication	Mesenchymal Stem Cells
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press
Pages	45-57
Number of pages	13
ISBN (Print)	9781588297716
DOIs	https://doi.org/10.1007/978-1-60327-169-1_3
Publication status	Published or Issued - 2008

Publication series

Name	Methods in Molecular Biology
Volume	449
ISSN (Print)	1064-3745

Keywords

Bone marrow stromal stem cells
Fluorescence activated cell sorting
Magnetic activated cell sorting
Mesenchymal stem cells

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-60327-169-1_3

Cite this

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A method to isolate and purify human bone marrow stromal stem cells

Abstract

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Keywords

ASJC Scopus subject areas

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