TY - CHAP
T1 - A method to isolate, purify, and characterize human periodontal ligament stem cells
AU - Mrozik, Krzysztof
AU - Gronthos, Stan
AU - Shi, Songtao
AU - Bartold, P. Mark
N1 - Publisher Copyright:
© Springer Science+Business Media LLC 2017.
PY - 2017
Y1 - 2017
N2 - Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1). Although no single antibody demonstrates specificity for MSCs, isolation based on expression of individual markers results in homogenous preparations of PDLSCs. Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast-, and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo, are also described.
AB - Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1). Although no single antibody demonstrates specificity for MSCs, isolation based on expression of individual markers results in homogenous preparations of PDLSCs. Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast-, and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo, are also described.
KW - Adherence isolation
KW - Differentiation potential
KW - Immunomagnetic isolation
KW - Mesenchymal stem cells
KW - Periodontal ligament stem cells
UR - https://www.scopus.com/pages/publications/85005938318
U2 - 10.1007/978-1-4939-6685-1_24
DO - 10.1007/978-1-4939-6685-1_24
M3 - Chapter
C2 - 27924608
AN - SCOPUS:85005938318
T3 - Methods in Molecular Biology
SP - 413
EP - 427
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -