Absence of hyaluronidase in cultured human skin fibroblasts

Bradley Arbogast; John J. Hopwood; Albert Dorfman

doi:10.1016/0006-291X(75)90326-5

Absence of hyaluronidase in cultured human skin fibroblasts

Bradley Arbogast, John J. Hopwood, Albert Dorfman

Hopwood Centre For Neurobiology

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The in vitro degradation of [³⁵S]chondroitin sulfate was investigated in human fibroblasts and rat liver. In rat liver, preparations of chondroitin sulfate were shown to be degraded by the concerted action of endoglycosidase and exoglycosidases. However, with human skin fibroblast preparations, hyaluronidase activity was not detected and chondroitin sulfate was degraded by exoglycosidase action.

Original language	English
Pages (from-to)	376-382
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	67
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(75)90326-5
Publication status	Published or Issued - 3 Nov 1975

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Absence of hyaluronidase in cultured human skin fibroblasts",

abstract = "The in vitro degradation of [35S]chondroitin sulfate was investigated in human fibroblasts and rat liver. In rat liver, preparations of chondroitin sulfate were shown to be degraded by the concerted action of endoglycosidase and exoglycosidases. However, with human skin fibroblast preparations, hyaluronidase activity was not detected and chondroitin sulfate was degraded by exoglycosidase action.",

author = "Bradley Arbogast and Hopwood, \{John J.\} and Albert Dorfman",

note = "Funding Information: by USPHS Grant Kennedy Fellow",

year = "1975",

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AU - Arbogast, Bradley

AU - Hopwood, John J.

AU - Dorfman, Albert

N1 - Funding Information: by USPHS Grant Kennedy Fellow

PY - 1975/11/3

Y1 - 1975/11/3

N2 - The in vitro degradation of [35S]chondroitin sulfate was investigated in human fibroblasts and rat liver. In rat liver, preparations of chondroitin sulfate were shown to be degraded by the concerted action of endoglycosidase and exoglycosidases. However, with human skin fibroblast preparations, hyaluronidase activity was not detected and chondroitin sulfate was degraded by exoglycosidase action.

AB - The in vitro degradation of [35S]chondroitin sulfate was investigated in human fibroblasts and rat liver. In rat liver, preparations of chondroitin sulfate were shown to be degraded by the concerted action of endoglycosidase and exoglycosidases. However, with human skin fibroblast preparations, hyaluronidase activity was not detected and chondroitin sulfate was degraded by exoglycosidase action.

UR - https://www.scopus.com/pages/publications/0016809592

U2 - 10.1016/0006-291X(75)90326-5

DO - 10.1016/0006-291X(75)90326-5

M3 - Article

C2 - 1201029

AN - SCOPUS:0016809592

SN - 0006-291X

VL - 67

SP - 376

EP - 382

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Absence of hyaluronidase in cultured human skin fibroblasts

Abstract

ASJC Scopus subject areas

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