AMPK and ACC phosphorylation: Effect of leptin, muscle fibre type and obesity

A. Janovská, G. Hatzinikolas, V. Staikopoulos, J. McInerney, M. Mano, G. A. Wittert

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Leptin stimulates fatty acid oxidation via the phosphorylation of AMPK (AMP-activated protein kinase) and ACC (acetyl-CoA carboxylase). Obesity is associated with resistance to the effects of leptin. We determined the action of leptin on AMPKα and ACCβ phosphorylation and lipid metabolism in soleus (SOL) and extensor digitorum longus (EDL) muscles from lean and obese Wistar rats after 1 and 100 nM leptin. Both leptin doses stimulated phosphorylation of AMPKα and ACCβ (P ≤ 0.05) only in EDL muscles from lean animals. Malonyl-CoA levels were decreased in EDL muscles from lean animals after 1 and 100 nM leptin and significantly after 100 nM leptin in obese animals (P ≤ 0.05). Long-chain fatty acyl-CoA concentrations were decreased in EDL muscles from both phenotypes after 100 nM leptin. AMPK activation by leptin occurred independently of energy-related metabolites. These data demonstrate that the leptin effect on AMPKα and ACCβ is muscle fibre type dependent and fails in diet-induced obesity. Crown

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume284
Issue number1-2
DOIs
Publication statusPublished or Issued - 12 Mar 2008
Externally publishedYes

Keywords

  • ACC
  • AMPK
  • LCFA-CoA
  • Leptin
  • Obesity
  • Skeletal muscle

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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