An alternative to cylinder drums for entering supplies and maintaining germ-free rodent isolators

Samay Trec, Mariah De Virgilio, Chris Christou

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The cost of supply cylinders can be expensive for smaller gnotobiotic facilities on strict budgets. Here, we present an alternative approach to enter supplies into germ-free isolators that does not require large cylinder drums (supply cylinders). We describe procedures for autoclaving double-bagged packs of supplies, sealing the packs aseptically into zip lock bags in biosafety cabinet, and entering supply packages into isolators. Our gnotobiotic facility has been using this protocol since 2018 and has been effective in maintaining germ-free status.

Original languageEnglish
Article number101511
JournalSTAR Protocols
Issue number3
Publication statusPublished or Issued - 16 Sept 2022


  • Microbiology
  • Model Organisms

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this