Abstract
Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel.
Original language | English |
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Pages (from-to) | 387-390 |
Number of pages | 4 |
Journal | BBA - Biomembranes |
Volume | 730 |
Issue number | 2 |
DOIs | |
Publication status | Published or Issued - 5 May 1983 |
Externally published | Yes |
Keywords
- (Ps. aeruginosa)
- Anion selectivity
- Channel formation
- Lipid bilayer
- Membrane reconstitution
- Outer membrane
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology