An anion-selective channel from the Pseudomonas aeruginosa outer membrane

Roland Benz; Michaela Gimple; Keith Poole; Robert E.W. Hancock

doi:10.1016/0005-2736(83)90356-5

An anion-selective channel from the Pseudomonas aeruginosa outer membrane

Roland Benz, Michaela Gimple, Keith Poole, Robert E.W. Hancock

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel.

Original language	English
Pages (from-to)	387-390
Number of pages	4
Journal	BBA - Biomembranes
Volume	730
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(83)90356-5
Publication status	Published or Issued - 5 May 1983
Externally published	Yes

Keywords

(Ps. aeruginosa)
Anion selectivity
Channel formation
Lipid bilayer
Membrane reconstitution
Outer membrane

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(83)90356-5

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title = "An anion-selective channel from the Pseudomonas aeruginosa outer membrane",

abstract = "Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel.",

keywords = "(Ps. aeruginosa), Anion selectivity, Channel formation, Lipid bilayer, Membrane reconstitution, Outer membrane",

author = "Roland Benz and Michaela Gimple and Keith Poole and Hancock, \{Robert E.W.\}",

note = "Funding Information: This work was supported by grants of the Deutsche Forschungsgemeinschaft (Sonderfor-schungsbereich 138 and Be 865/' 1-1) and by grants to R.E.W.H. from the National Scientific and Engineering Research Council of Canada and the Canadian Cystic Fibrosis Foundation.",

year = "1983",

month = may,

day = "5",

doi = "10.1016/0005-2736(83)90356-5",

language = "English",

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pages = "387--390",

journal = "BBA - Biomembranes",

issn = "0005-2736",

publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - An anion-selective channel from the Pseudomonas aeruginosa outer membrane

AU - Benz, Roland

AU - Gimple, Michaela

AU - Poole, Keith

AU - Hancock, Robert E.W.

N1 - Funding Information: This work was supported by grants of the Deutsche Forschungsgemeinschaft (Sonderfor-schungsbereich 138 and Be 865/' 1-1) and by grants to R.E.W.H. from the National Scientific and Engineering Research Council of Canada and the Canadian Cystic Fibrosis Foundation.

PY - 1983/5/5

Y1 - 1983/5/5

N2 - Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel.

AB - Protein P from Pseudomonas aeruginosa outer membrane was reconstituted in lipid bilayer membranes from diphytanoylphosphatidylcholine. The reconstitution resulted in the formation of anion-selective channels with a conductance of 160 pS for 0.1 M chloride solution. The channels were at least 100-times more selective for anions than for cations as judged from zero-current membrane potentials. The single-channel conductance was dependent on the size of the different anions and saturated at higher salt concentrations suggesting single ion occupancy of the protein P channel.

KW - (Ps. aeruginosa)

KW - Anion selectivity

KW - Channel formation

KW - Lipid bilayer

KW - Membrane reconstitution

KW - Outer membrane

UR - https://www.scopus.com/pages/publications/0020560434

U2 - 10.1016/0005-2736(83)90356-5

DO - 10.1016/0005-2736(83)90356-5

M3 - Article

AN - SCOPUS:0020560434

SN - 0005-2736

VL - 730

SP - 387

EP - 390

JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 2

ER -

An anion-selective channel from the Pseudomonas aeruginosa outer membrane

Abstract

Keywords

ASJC Scopus subject areas

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