An early inflammatory gene profile in visceral adipose tissue in children

Charmaine S. Tam; Leonie Heilbronn; Corneliu Henegar; Melanie Wong; Christopher T. Cowell; Mark J. Cowley; Warren Kaplan; Karine Clément; Louise A. Baur

doi:10.3109/17477166.2011.575152

An early inflammatory gene profile in visceral adipose tissue in children

Charmaine S. Tam, Leonie Heilbronn, Corneliu Henegar, Melanie Wong, Christopher T. Cowell, Mark J. Cowley, Warren Kaplan, Karine Clément, Louise A. Baur

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

The aim of this study was to characterize expression profiles of visceral and subcutaneous adipose tissue in children. Adipose tissue samples were collected from children having elective surgery (n = 71, [54 boys], 6.0 ± 4.3 years). Affymetrix microarrays (n = 20) were performed to characterize the functional profile and identify genes of interest in adipose tissue. Visceral adipose tissue had an overrepresentation of Gene Ontology themes related to immune and inflammatory responses and subcutaneous adipose tissue had an overrepresentation of themes related to adipocyte growth and development. Likewise, qPCR performed in the whole cohort showed a 30-fold increase in haptoglobin (P = 0.005), 7-fold increase in IL-10 (P < 0.001), 8-fold decrease in VEGF (P = 0.01) and a 28-fold decrease in TBOX15 (P < 0.001) in visceral compared to subcutaneous adipose tissue. The inflammatory pattern in visceral adipose tissue may represent an early stage of the adverse effects of this depot, and combined with chronic obesity, may contribute to increased metabolic and cardiovascular risk.

Original language	English
Pages (from-to)	e360-e363
Journal	International Journal of Pediatric Obesity
Volume	6
Issue number	2 -2
DOIs	https://doi.org/10.3109/17477166.2011.575152
Publication status	Published or Issued - Jun 2011

Keywords

Adipose tissue
Children
Inflammation
Visceral

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Health Policy
Nutrition and Dietetics
Public Health, Environmental and Occupational Health

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10.3109/17477166.2011.575152

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title = "An early inflammatory gene profile in visceral adipose tissue in children",

abstract = "The aim of this study was to characterize expression profiles of visceral and subcutaneous adipose tissue in children. Adipose tissue samples were collected from children having elective surgery (n = 71, [54 boys], 6.0 ± 4.3 years). Affymetrix microarrays (n = 20) were performed to characterize the functional profile and identify genes of interest in adipose tissue. Visceral adipose tissue had an overrepresentation of Gene Ontology themes related to immune and inflammatory responses and subcutaneous adipose tissue had an overrepresentation of themes related to adipocyte growth and development. Likewise, qPCR performed in the whole cohort showed a 30-fold increase in haptoglobin (P = 0.005), 7-fold increase in IL-10 (P < 0.001), 8-fold decrease in VEGF (P = 0.01) and a 28-fold decrease in TBOX15 (P < 0.001) in visceral compared to subcutaneous adipose tissue. The inflammatory pattern in visceral adipose tissue may represent an early stage of the adverse effects of this depot, and combined with chronic obesity, may contribute to increased metabolic and cardiovascular risk.",

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AU - Cowley, Mark J.

AU - Kaplan, Warren

AU - Clément, Karine

AU - Baur, Louise A.

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An early inflammatory gene profile in visceral adipose tissue in children

Abstract

Keywords

ASJC Scopus subject areas

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