Abstract
Abstract Innate defense regulator (IDR) peptides are a class of immunomodulators which enhance and modulate host innate immune responses against microbial pathogens. While IDR-mediated protection against a range of bacterial pathogens is dependent on enhanced monocyte recruitment to the site of infection, the mechanisms through which they increase monocyte trafficking remain unclear. In this study, anti-infective peptide IDR-1002 was shown to enhance monocyte chemotaxis towards chemokines CCL3 and CCL5. This enhancement correlated with the selective upregulation of CCR5 surface expression by peptide-treated monocytes. It was found that IDR-1002 enhancement of monocyte chemotaxis was fully dependent on CCR5 function. Furthermore, IDR-1002 enhanced chemokine-induced monocyte p38 MAPK phosphorylation in a CCR5-dependent fashion. Overall, these results indicate that peptide IDR-1002 can selectively influence monocyte recruitment by host chemokines through the regulation of chemokine receptors.
| Original language | English |
|---|---|
| Article number | 34248 |
| Pages (from-to) | 800-806 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 464 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published or Issued - 7 Aug 2015 |
| Externally published | Yes |
Keywords
- Chemokine receptors
- Chemokines
- Immunomodulation
- Innate defense regulators
- Monocytes
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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