ATP-dependent copper transport by the Menkes protein in membrane vesicles isolated from cultured Chinese hamster ovary cells

Ilia Voskoboinik, Hilary Brooks, Suzanne Smith, Peiyan Shen, James Camakaris

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

The Menkes (MNK) protein is a vital component of copper homeostasis in mammalian cells. In this paper we provide the first biochemical evidence that the MNK protein functions as a copper-translocating P-type ATPase in mammalian cells. The enzyme activity in membrane vesicles prepared from Chinese hamster ovary cells overexpressing MNK was ATP-dependent, correlated with the amount of MNK and followed Michaelis-Menten kinetics with respect to copper. The copper transport was observed only under reducing conditions suggesting MNK transports Cu(I). This study opens the way to detailed structure-function studies and assessment of functional MNK derived from patients with Menkes disease. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalFEBS Letters
Volume435
Issue number2-3
DOIs
Publication statusPublished or Issued - 18 Sep 1998
Externally publishedYes

Keywords

  • Copper
  • Copper transport
  • Menkes disease
  • P-type ATPase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this