TY - JOUR
T1 - Atriplex hortensis var. 'rubra' extracts and purified amaranthin-type pigments reduce oxidative stress and inflammatory response in LPS-stimulated RAW264.7 cells
AU - Kumorkiewicz-Jamro, Agnieszka
AU - Pachulicz, River J.
AU - Fitter, Stephen
AU - Górska, Renata
AU - Duggan, Jvaughn
AU - Vandyke, Kate
AU - Pukala, Tara L.
AU - Wybraniec, Sławomir
AU - Zannettino, Andrew C.W.
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2025/1/1
Y1 - 2025/1/1
N2 - The use of direct injection ion mobility mass spectrometry (DI-IM-MS) to detect and identify betacyanin pigments in A. hortensis ‘rubra’ extracts was explored for the first time, with results compared to conventional LC-MS/MS analysis. The anti-inflammatory activities of leaf and seed extracts, alongside purified amaranthin and celosianin pigments, were investigated using a model of lipopolysaccharide (LPS)-activated murine macrophages. Extracts and purified pigments significantly inhibited the production of prostaglandin E2 and NO by up to 90% and 70%, respectively, and reduced the expression of Il6, Il1b, Nos2, and Cox2. Leaf and seed extracts also decreased secretion of Il6 and Il1b cytokines and reduced protein levels of Nos2 and Cox2. Furthermore, extracts and purified pigments demonstrated potent dose-dependent radical scavenging activity in a cellular antioxidant activity assay (CAA) without any cytotoxic effects. Our research highlights the promising biological potential of edible, climate-resilient A. hortensis ‘rubra’ as a valuable source of bioactive compounds.
AB - The use of direct injection ion mobility mass spectrometry (DI-IM-MS) to detect and identify betacyanin pigments in A. hortensis ‘rubra’ extracts was explored for the first time, with results compared to conventional LC-MS/MS analysis. The anti-inflammatory activities of leaf and seed extracts, alongside purified amaranthin and celosianin pigments, were investigated using a model of lipopolysaccharide (LPS)-activated murine macrophages. Extracts and purified pigments significantly inhibited the production of prostaglandin E2 and NO by up to 90% and 70%, respectively, and reduced the expression of Il6, Il1b, Nos2, and Cox2. Leaf and seed extracts also decreased secretion of Il6 and Il1b cytokines and reduced protein levels of Nos2 and Cox2. Furthermore, extracts and purified pigments demonstrated potent dose-dependent radical scavenging activity in a cellular antioxidant activity assay (CAA) without any cytotoxic effects. Our research highlights the promising biological potential of edible, climate-resilient A. hortensis ‘rubra’ as a valuable source of bioactive compounds.
KW - Anti-inflammatory activity
KW - Antioxidant activity
KW - Atriplex hortensis ‘rubra’
KW - Betalains
KW - Ion mobility-mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=85202210951&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2024.140920
DO - 10.1016/j.foodchem.2024.140920
M3 - Article
AN - SCOPUS:85202210951
SN - 0308-8146
VL - 462
JO - Food Chemistry
JF - Food Chemistry
M1 - 140920
ER -