TY - JOUR
T1 - Autophagy across tissues of aging mice
AU - Carosi, Julian M.
AU - Martin, Alexis
AU - Hein, Leanne K.
AU - Hassiotis, Sofia
AU - Hattersley, Kathryn J.
AU - Turner, Bradley J.
AU - Fourrier, Célia
AU - Bensalem, Julien
AU - Sargeant, Timothy J.
N1 - Publisher Copyright:
© 2025 Carosi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2025/6/4
Y1 - 2025/6/4
N2 - Autophagy is a ‘waste-disposal’ pathway that protects against age-related pathology. It is widely accepted that autophagy declines with age, yet the role that sex and diet-related obesity play during aging remain unknown. Here, we present the most comprehensive in vivo study of autophagic flux to date. We employed transgenic mice overexpressing tandem-florescent LC3B (RFP-GFP-LC3B) to measure autophagic flux in the blood (PBMCs), heart, and motor cortex neurons of aging mice that were fed regular chow or a high-fat diet for 6-, 12- or 18-months. In male mice, aging decreased autophagic flux in the heart, increased it in the blood, and had no effect in motor cortex neurons. Age-dependent changes autophagic flux were less pronounced in female mice. High-fat diet influenced autophagic flux in the blood and heart of male but not female mice. Overall, we uncovered sexual dimorphisms that underpin how autophagy changes with age across different tissues and in response to a high-fat diet.
AB - Autophagy is a ‘waste-disposal’ pathway that protects against age-related pathology. It is widely accepted that autophagy declines with age, yet the role that sex and diet-related obesity play during aging remain unknown. Here, we present the most comprehensive in vivo study of autophagic flux to date. We employed transgenic mice overexpressing tandem-florescent LC3B (RFP-GFP-LC3B) to measure autophagic flux in the blood (PBMCs), heart, and motor cortex neurons of aging mice that were fed regular chow or a high-fat diet for 6-, 12- or 18-months. In male mice, aging decreased autophagic flux in the heart, increased it in the blood, and had no effect in motor cortex neurons. Age-dependent changes autophagic flux were less pronounced in female mice. High-fat diet influenced autophagic flux in the blood and heart of male but not female mice. Overall, we uncovered sexual dimorphisms that underpin how autophagy changes with age across different tissues and in response to a high-fat diet.
UR - http://www.scopus.com/inward/record.url?scp=105007363357&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0325505
DO - 10.1371/journal.pone.0325505
M3 - Article
AN - SCOPUS:105007363357
SN - 1932-6203
VL - 20
JO - PloS one
JF - PloS one
IS - 6 June
M1 - e0325505
ER -