Betalains isolated from underexploited wild plant Atriplex hortensis var. rubra L. exert antioxidant and cardioprotective activity against H9c2 cells

Agnieszka Kumorkiewicz-Jamro, Renata Górska, Małgorzata Krok-Borkowicz, Katarzyna Reczyńska-Kolman, Przemysław Mielczarek, Łukasz Popenda, Aneta Spórna-Kucab, Anna Tekieli, Elżbieta Pamuła, Sławomir Wybraniec

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1 Citation (Scopus)


Atriplex hortensis var. rubra L. extracts prepared from leaves, seeds with sheaths, and stems were characterized for betalainic profiles by spectrophotometry, LC-DAD-ESI-MS/MS and LC-Orbitrap-MS techniques. The presence of 12 betacyanins in the extracts was strongly correlated with high antioxidant activity measured by ABTS, FRAP, and ORAC assays. Comparative assessment between samples indicated the highest potential for celosianin and amaranthin (IC50 21.5 and 32.2 μg/ml, respectively). The chemical structure of celosianin was elucidated for the first time by complete 1D and 2D NMR analysis. Our findings also demonstrate that betalain-rich A. hortensis extracts and purified pigments (amaranthin and celosianin) do not induce cytotoxicity in a wide concentration range in rat cardiomyocytes model (up to 100 μg/ml for extracts and 1 mg/ml for pigments). Furthermore, tested samples effectively protect H9c2 cells from H2O2-induced cell death and prevent from apoptosis induced by Paclitaxel. The effects were observed at sample concentrations between 0.1 and 10 μg/ml.

Original languageEnglish
Article number135641
JournalFood Chemistry
Publication statusPublished or Issued - 7 Feb 2023
Externally publishedYes


  • Amaranthaceae family
  • Amaranthin
  • Antioxidant activity
  • Atriplex hortensis var. rubra
  • Betalains
  • Cardioprotective activity
  • Celosianin
  • Natural pigments

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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