TY - JOUR
T1 - Betalains isolated from underexploited wild plant Atriplex hortensis var. rubra L. exert antioxidant and cardioprotective activity against H9c2 cells
AU - Kumorkiewicz-Jamro, Agnieszka
AU - Górska, Renata
AU - Krok-Borkowicz, Małgorzata
AU - Reczyńska-Kolman, Katarzyna
AU - Mielczarek, Przemysław
AU - Popenda, Łukasz
AU - Spórna-Kucab, Aneta
AU - Tekieli, Anna
AU - Pamuła, Elżbieta
AU - Wybraniec, Sławomir
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2023/7/15
Y1 - 2023/7/15
N2 - Atriplex hortensis var. rubra L. extracts prepared from leaves, seeds with sheaths, and stems were characterized for betalainic profiles by spectrophotometry, LC-DAD-ESI-MS/MS and LC-Orbitrap-MS techniques. The presence of 12 betacyanins in the extracts was strongly correlated with high antioxidant activity measured by ABTS, FRAP, and ORAC assays. Comparative assessment between samples indicated the highest potential for celosianin and amaranthin (IC50 21.5 and 32.2 μg/ml, respectively). The chemical structure of celosianin was elucidated for the first time by complete 1D and 2D NMR analysis. Our findings also demonstrate that betalain-rich A. hortensis extracts and purified pigments (amaranthin and celosianin) do not induce cytotoxicity in a wide concentration range in rat cardiomyocytes model (up to 100 μg/ml for extracts and 1 mg/ml for pigments). Furthermore, tested samples effectively protect H9c2 cells from H2O2-induced cell death and prevent from apoptosis induced by Paclitaxel. The effects were observed at sample concentrations between 0.1 and 10 μg/ml.
AB - Atriplex hortensis var. rubra L. extracts prepared from leaves, seeds with sheaths, and stems were characterized for betalainic profiles by spectrophotometry, LC-DAD-ESI-MS/MS and LC-Orbitrap-MS techniques. The presence of 12 betacyanins in the extracts was strongly correlated with high antioxidant activity measured by ABTS, FRAP, and ORAC assays. Comparative assessment between samples indicated the highest potential for celosianin and amaranthin (IC50 21.5 and 32.2 μg/ml, respectively). The chemical structure of celosianin was elucidated for the first time by complete 1D and 2D NMR analysis. Our findings also demonstrate that betalain-rich A. hortensis extracts and purified pigments (amaranthin and celosianin) do not induce cytotoxicity in a wide concentration range in rat cardiomyocytes model (up to 100 μg/ml for extracts and 1 mg/ml for pigments). Furthermore, tested samples effectively protect H9c2 cells from H2O2-induced cell death and prevent from apoptosis induced by Paclitaxel. The effects were observed at sample concentrations between 0.1 and 10 μg/ml.
KW - Amaranthaceae family
KW - Amaranthin
KW - Antioxidant activity
KW - Atriplex hortensis var. rubra
KW - Betalains
KW - Cardioprotective activity
KW - Celosianin
KW - Natural pigments
UR - http://www.scopus.com/inward/record.url?scp=85148323311&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2023.135641
DO - 10.1016/j.foodchem.2023.135641
M3 - Article
C2 - 36809729
AN - SCOPUS:85148323311
SN - 0308-8146
VL - 414
JO - Food Chemistry
JF - Food Chemistry
M1 - 135641
ER -