Biosensors for detecting stress in developing embryos

Malcolm S. Purdey; Avishkar Saini; Hanna J. McLennan; Benjamin J. Pullen; Erik P. Schartner; Melanie L. Sutton-Mcdowall; Jeremy G. Thompson; Tanya M. Monro; Stephen J. Nicholls; Andrew D. Abell

doi:10.1117/12.2244645

Biosensors for detecting stress in developing embryos

Malcolm S. Purdey, Avishkar Saini, Hanna J. McLennan, Benjamin J. Pullen, Erik P. Schartner, Melanie L. Sutton-Mcdowall, Jeremy G. Thompson, Tanya M. Monro, Stephen J. Nicholls, Andrew D. Abell

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Citation (Scopus)

Abstract

Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H₂O₂) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H₂O₂ using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H₂O₂ to non-invasively detect H₂O₂ near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H₂O₂ and pH that is functional at biologically concentrations of H₂O₂ and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.

Original language	English
Title of host publication	SPIE BioPhotonics Australasia
Editors	Ewa M. Goldys, Mark R. Hutchinson
Publisher	SPIE
ISBN (Electronic)	9781510604346
DOIs	https://doi.org/10.1117/12.2244645
Publication status	Published or Issued - 2016
Event	SPIE BioPhotonics Australasia - Adelaide, Australia Duration: 17 Oct 2016 → 19 Oct 2016

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10013
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Other

Other	SPIE BioPhotonics Australasia
Country/Territory	Australia
City	Adelaide
Period	17/10/16 → 19/10/16

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.2244645

Cite this

Purdey, M. S., Saini, A., McLennan, H. J., Pullen, B. J., Schartner, E. P., Sutton-Mcdowall, M. L., Thompson, J. G., Monro, T. M., Nicholls, S. J., & Abell, A. D. (2016). Biosensors for detecting stress in developing embryos. In E. M. Goldys, & M. R. Hutchinson (Eds.), SPIE BioPhotonics Australasia Article 100130B (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10013). SPIE. https://doi.org/10.1117/12.2244645

@inproceedings{ba4fbecb86ff4270b558e5a1e8c05f9d,

title = "Biosensors for detecting stress in developing embryos",

abstract = "Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H2O2) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H2O2 using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H2O2 to non-invasively detect H2O2 near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H2O2 and pH that is functional at biologically concentrations of H2O2 and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.",

author = "Purdey, {Malcolm S.} and Avishkar Saini and McLennan, {Hanna J.} and Pullen, {Benjamin J.} and Schartner, {Erik P.} and Sutton-Mcdowall, {Melanie L.} and Thompson, {Jeremy G.} and Monro, {Tanya M.} and Nicholls, {Stephen J.} and Abell, {Andrew D.}",

note = "Publisher Copyright: {\textcopyright} 2016 SPIE.; SPIE BioPhotonics Australasia ; Conference date: 17-10-2016 Through 19-10-2016",

year = "2016",

doi = "10.1117/12.2244645",

language = "English",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Goldys, {Ewa M.} and Hutchinson, {Mark R.}",

booktitle = "SPIE BioPhotonics Australasia",

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Purdey, MS, Saini, A, McLennan, HJ, Pullen, BJ, Schartner, EP, Sutton-Mcdowall, ML, Thompson, JG, Monro, TM, Nicholls, SJ & Abell, AD 2016, Biosensors for detecting stress in developing embryos. in EM Goldys & MR Hutchinson (eds), SPIE BioPhotonics Australasia., 100130B, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10013, SPIE, SPIE BioPhotonics Australasia, Adelaide, Australia, 17/10/16. https://doi.org/10.1117/12.2244645

Biosensors for detecting stress in developing embryos. / Purdey, Malcolm S.; Saini, Avishkar; McLennan, Hanna J. et al.
SPIE BioPhotonics Australasia. ed. / Ewa M. Goldys; Mark R. Hutchinson. SPIE, 2016. 100130B (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Purdey, Malcolm S.

AU - Saini, Avishkar

AU - McLennan, Hanna J.

AU - Pullen, Benjamin J.

AU - Schartner, Erik P.

AU - Sutton-Mcdowall, Melanie L.

AU - Thompson, Jeremy G.

AU - Monro, Tanya M.

AU - Nicholls, Stephen J.

AU - Abell, Andrew D.

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N2 - Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H2O2) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H2O2 using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H2O2 to non-invasively detect H2O2 near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H2O2 and pH that is functional at biologically concentrations of H2O2 and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.

AB - Reactive Oxygen Species (ROS) cause DNA damage and defective function in sperm and also affects the developmental competence of embryos. It is therefore critical to monitor ROS in sperm, oocytes and developing embryos. In particular, hydrogen peroxide (H2O2) is a ROS important to normal cell function and signalling as well as its role in oxidative stress. Here we report the development of a fluorescent sensor for H2O2 using carboxyperoxyfluor-1 (CPF1) in solution and attached to a glass slide or multi-mode optical fibre. CPF1 increases in fluorescence upon reaction with H2O2 to non-invasively detect H2O2 near developing embryos. These probes are constructed by immobilising CPF1 to the optical fibre tip a polyacrylamide layer. Also reported is a new dual optical fibre sensor for detecting both H2O2 and pH that is functional at biologically concentrations of H2O2 and can sense pH to 0.1 units. This research shows promise for the use of optical fibre sensors for monitoring the health of developing embryos. Furthermore, these sensors are applicable for use beyond embryos such as detecting stress in endothelial cells involved in cardiovascular dysfunction.

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M3 - Conference contribution

AN - SCOPUS:85011422790

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - SPIE BioPhotonics Australasia

A2 - Goldys, Ewa M.

A2 - Hutchinson, Mark R.

PB - SPIE

T2 - SPIE BioPhotonics Australasia

Y2 - 17 October 2016 through 19 October 2016

ER -

Biosensors for detecting stress in developing embryos

Abstract

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