TY - JOUR
T1 - Biosynthesis and metabolism of indol-3yl-acetic acid
T2 - III. Partial purification and properties of a tryptamine-forming l-tryptophan dbcarboxylase from tomato shoots
AU - Gibson, R. A.
AU - Barrett, Geraldine
AU - Wightman, F.
N1 - Funding Information:
ACKNOWLEDGEMENT This investigation was supported by a grant-in-aid of research awarded to one of us (F. W.) by the National Research Council of Canada for which we are most grateful.
PY - 1972/8
Y1 - 1972/8
N2 - A tryptamine-forming, L-tryptophan decarboxylase (E.C. 4.1.1.27) from tomato shoots, has been partially purified and characterized. The properties of the enzyme were compared with those of tryptophan transaminase isolated from the same tissue, and separation of these two enzymes by ammonium sulphate fractionation clearly demonstrated that tryptamine formation was due to the activity of the decarboxylase enzyme. Tryptophan decarboxylase was found to be pyridoxal phosphate dependent and appeared to have substrate affinities different from those of 5-hydroxytryptophan decarboxylase (E.C. 4.1.1.28) found in animal tissue. The importance of tryptophan decarboxylase in the biosynthesis of indol-3yl-acetic acid is discussed.
AB - A tryptamine-forming, L-tryptophan decarboxylase (E.C. 4.1.1.27) from tomato shoots, has been partially purified and characterized. The properties of the enzyme were compared with those of tryptophan transaminase isolated from the same tissue, and separation of these two enzymes by ammonium sulphate fractionation clearly demonstrated that tryptamine formation was due to the activity of the decarboxylase enzyme. Tryptophan decarboxylase was found to be pyridoxal phosphate dependent and appeared to have substrate affinities different from those of 5-hydroxytryptophan decarboxylase (E.C. 4.1.1.28) found in animal tissue. The importance of tryptophan decarboxylase in the biosynthesis of indol-3yl-acetic acid is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0343248665&partnerID=8YFLogxK
U2 - 10.1093/jxb/23.3.775
DO - 10.1093/jxb/23.3.775
M3 - Article
AN - SCOPUS:0343248665
SN - 0022-0957
VL - 23
SP - 775
EP - 786
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 3
ER -