Cellulases (CM Cases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost

Lungisa Mayende; Brendan S. Wilhelmi; Brett I. Pletschke

doi:10.1016/j.soilbio.2006.03.019

Cellulases (CM Cases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost

Lungisa Mayende, Brendan S. Wilhelmi, Brett I. Pletschke

Research output: Contribution to journal › Article › peer-review

71 Citations (Scopus)

Abstract

In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml ^-1 min^-1. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml^-1 min^-1. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.

Original language	English
Pages (from-to)	2963-2966
Number of pages	4
Journal	Soil Biology and Biochemistry
Volume	38
Issue number	9
DOIs	https://doi.org/10.1016/j.soilbio.2006.03.019
Publication status	Published or Issued - Sept 2006
Externally published	Yes

Keywords

Cellulases
Compost
Polyphenol oxidases
Thermophiles

ASJC Scopus subject areas

Microbiology
Soil Science

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10.1016/j.soilbio.2006.03.019

Cite this

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title = "Cellulases (CM Cases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost",

abstract = "In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml -1 min-1. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml-1 min-1. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.",

keywords = "Cellulases, Compost, Polyphenol oxidases, Thermophiles",

author = "Lungisa Mayende and Wilhelmi, {Brendan S.} and Pletschke, {Brett I.}",

note = "Funding Information: We acknowledge SASOL and the National Research Foundation of South Africa for financial support. ",

year = "2006",

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doi = "10.1016/j.soilbio.2006.03.019",

language = "English",

volume = "38",

pages = "2963--2966",

journal = "Soil Biology and Biochemistry",

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TY - JOUR

T1 - Cellulases (CM Cases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost

AU - Mayende, Lungisa

AU - Wilhelmi, Brendan S.

AU - Pletschke, Brett I.

N1 - Funding Information: We acknowledge SASOL and the National Research Foundation of South Africa for financial support.

PY - 2006/9

Y1 - 2006/9

N2 - In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml -1 min-1. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml-1 min-1. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.

AB - In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 °C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 °C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml -1 min-1. Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml-1 min-1. Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis.

KW - Cellulases

KW - Compost

KW - Polyphenol oxidases

KW - Thermophiles

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U2 - 10.1016/j.soilbio.2006.03.019

DO - 10.1016/j.soilbio.2006.03.019

M3 - Article

AN - SCOPUS:34548129892

SN - 0038-0717

VL - 38

SP - 2963

EP - 2966

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

IS - 9

ER -

Cellulases (CM Cases) and polyphenol oxidases from thermophilic Bacillus spp. isolated from compost

Abstract

Keywords

ASJC Scopus subject areas

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