TY - JOUR
T1 - Cereal based diets modulate some markers of oxidative stress and inflammation in lean and obese Zucker rats
AU - Belobrajdic, Damien P.
AU - Lam, Yan Y.
AU - Mano, Mark
AU - Wittert, Gary A.
AU - Bird, Anthony R.
N1 - Funding Information:
We wish to thank Jean Wei, Julie Dallimore, Debbie Davies, Jennifer Giles, Brad Klingner, Sharyn Zrna, Jenny McInerney and Paul Orchard for excellent animal care and technical assistance. Amanda Box from the University of Adelaide for the supply of the Torrens barley for the diets. Financial support was provided by the CSIRO Food Futures Flagship.
PY - 2011
Y1 - 2011
N2 - Background: The potential of cereals with high antioxidant capacity for reducing oxidative stress and inflammation in obesity is unknown. This study investigated the impact of wheat bran, barley or a control diet (-cellulose) on the development of oxidative stress and inflammation in lean and obese Zucker rats. Methods. Seven wk old, lean and obese male Zucker rats (n = 8/group) were fed diets that contained wheat bran, barley or -cellulose (control). After 3 months on these diets, systolic blood pressure was measured and plasma was analysed for glucose, insulin, lipids, oxygen radical absorbance capacity (ORAC), malondialdehyde, glutathione peroxidase and adipokine concentration (leptin, adiponectin, interleukin (IL)-1, IL-6, TNF, plasminogen activator inhibitor (PAI)-1, monocyte chemotactic protein (MCP)-1). Adipokine secretion rates from visceral and subcutaneous adipose tissue explants were also determined. Results: Obese rats had higher body weight, systolic blood pressure and fasting blood lipids, glucose, insulin, leptin and IL-1 in comparison to lean rats, and these measures were not reduced by consumption of wheat bran or barley based diets. Serum ORAC tended to be higher in obese rats fed wheat bran and barley in comparison to control (p = 0.06). Obese rats had higher plasma malondialdehyde (p < 0.01) and lower plasma glutathione peroxidase concentration (p < 0.01) but these levels were not affected by diet type. PAI-1 was elevated in the plasma of obese rats, and the wheat bran diet in comparison to the control group reduced PAI-1 to levels seen in the lean rats (p < 0.05). These changes in circulating PAI-1 levels could not be explained by PAI-1 secretion rates from visceral or subcutaneous adipose tissue. Conclusions: A 3-month dietary intervention was sufficient for Zucker obese rats to develop oxidative stress and systemic inflammation. Cereal-based diets with moderate and high antioxidant capacity elicited modest improvements in indices of oxidative stress and inflammation.
AB - Background: The potential of cereals with high antioxidant capacity for reducing oxidative stress and inflammation in obesity is unknown. This study investigated the impact of wheat bran, barley or a control diet (-cellulose) on the development of oxidative stress and inflammation in lean and obese Zucker rats. Methods. Seven wk old, lean and obese male Zucker rats (n = 8/group) were fed diets that contained wheat bran, barley or -cellulose (control). After 3 months on these diets, systolic blood pressure was measured and plasma was analysed for glucose, insulin, lipids, oxygen radical absorbance capacity (ORAC), malondialdehyde, glutathione peroxidase and adipokine concentration (leptin, adiponectin, interleukin (IL)-1, IL-6, TNF, plasminogen activator inhibitor (PAI)-1, monocyte chemotactic protein (MCP)-1). Adipokine secretion rates from visceral and subcutaneous adipose tissue explants were also determined. Results: Obese rats had higher body weight, systolic blood pressure and fasting blood lipids, glucose, insulin, leptin and IL-1 in comparison to lean rats, and these measures were not reduced by consumption of wheat bran or barley based diets. Serum ORAC tended to be higher in obese rats fed wheat bran and barley in comparison to control (p = 0.06). Obese rats had higher plasma malondialdehyde (p < 0.01) and lower plasma glutathione peroxidase concentration (p < 0.01) but these levels were not affected by diet type. PAI-1 was elevated in the plasma of obese rats, and the wheat bran diet in comparison to the control group reduced PAI-1 to levels seen in the lean rats (p < 0.05). These changes in circulating PAI-1 levels could not be explained by PAI-1 secretion rates from visceral or subcutaneous adipose tissue. Conclusions: A 3-month dietary intervention was sufficient for Zucker obese rats to develop oxidative stress and systemic inflammation. Cereal-based diets with moderate and high antioxidant capacity elicited modest improvements in indices of oxidative stress and inflammation.
UR - http://www.scopus.com/inward/record.url?scp=79955425869&partnerID=8YFLogxK
U2 - 10.1186/1743-7075-8-27
DO - 10.1186/1743-7075-8-27
M3 - Article
C2 - 21535898
AN - SCOPUS:79955425869
SN - 1743-7075
VL - 8
JO - Nutrition and Metabolism
JF - Nutrition and Metabolism
M1 - 27
ER -