Colorimetric biosensor vesicles for biotechnological applications

Revital Halevy; Sofiya Kolusheva; Robert E.W. Hancock; Raz Jelinek

doi:10.1557/proc-724-n7.23

Colorimetric biosensor vesicles for biotechnological applications

Revital Halevy, Sofiya Kolusheva, Robert E.W. Hancock, Raz Jelinek

Research output: Contribution to journal › Conference article › peer-review

1 Citation (Scopus)

Abstract

Supramolecular assemblies of vesicles composed of natural lipids embedded in a matrix of polydiacetylene are shown to undergo blue-to-red color changes related to specific interactions between the lipids or recognition elements and biomolecules. The colorimetric transitions observed in the vesicles are due to structural modifications within the conjugated PDA polymer backbone. This colorimetric assay could serve as a membrane-mimicking environment for detection of interfacial processes, such as peptide-membrane interactions, antibody-antigen binding, enzymatic catalysis, and detection of physiological ions. The lipid/PDA mixed vesicles are robust and can be readily applied to diagnosis of physiological molecules and rapid screening of biological and chemical libraries.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	Materials Research Society Symposium - Proceedings
Volume	724
DOIs	https://doi.org/10.1557/proc-724-n7.23
Publication status	Published or Issued - 2002
Externally published	Yes
Event	Biological and Biomimetic Materials - Properties to Function - San Francisco, CA, United States Duration: 1 Apr 2002 → 5 Apr 2002

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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title = "Colorimetric biosensor vesicles for biotechnological applications",

abstract = "Supramolecular assemblies of vesicles composed of natural lipids embedded in a matrix of polydiacetylene are shown to undergo blue-to-red color changes related to specific interactions between the lipids or recognition elements and biomolecules. The colorimetric transitions observed in the vesicles are due to structural modifications within the conjugated PDA polymer backbone. This colorimetric assay could serve as a membrane-mimicking environment for detection of interfacial processes, such as peptide-membrane interactions, antibody-antigen binding, enzymatic catalysis, and detection of physiological ions. The lipid/PDA mixed vesicles are robust and can be readily applied to diagnosis of physiological molecules and rapid screening of biological and chemical libraries.",

author = "Revital Halevy and Sofiya Kolusheva and Hancock, {Robert E.W.} and Raz Jelinek",

year = "2002",

doi = "10.1557/proc-724-n7.23",

language = "English",

volume = "724",

pages = "153--158",

journal = "Materials Research Society Symposium - Proceedings",

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note = "Biological and Biomimetic Materials - Properties to Function ; Conference date: 01-04-2002 Through 05-04-2002",

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TY - JOUR

T1 - Colorimetric biosensor vesicles for biotechnological applications

AU - Halevy, Revital

AU - Kolusheva, Sofiya

AU - Hancock, Robert E.W.

AU - Jelinek, Raz

PY - 2002

Y1 - 2002

N2 - Supramolecular assemblies of vesicles composed of natural lipids embedded in a matrix of polydiacetylene are shown to undergo blue-to-red color changes related to specific interactions between the lipids or recognition elements and biomolecules. The colorimetric transitions observed in the vesicles are due to structural modifications within the conjugated PDA polymer backbone. This colorimetric assay could serve as a membrane-mimicking environment for detection of interfacial processes, such as peptide-membrane interactions, antibody-antigen binding, enzymatic catalysis, and detection of physiological ions. The lipid/PDA mixed vesicles are robust and can be readily applied to diagnosis of physiological molecules and rapid screening of biological and chemical libraries.

AB - Supramolecular assemblies of vesicles composed of natural lipids embedded in a matrix of polydiacetylene are shown to undergo blue-to-red color changes related to specific interactions between the lipids or recognition elements and biomolecules. The colorimetric transitions observed in the vesicles are due to structural modifications within the conjugated PDA polymer backbone. This colorimetric assay could serve as a membrane-mimicking environment for detection of interfacial processes, such as peptide-membrane interactions, antibody-antigen binding, enzymatic catalysis, and detection of physiological ions. The lipid/PDA mixed vesicles are robust and can be readily applied to diagnosis of physiological molecules and rapid screening of biological and chemical libraries.

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U2 - 10.1557/proc-724-n7.23

DO - 10.1557/proc-724-n7.23

M3 - Conference article

AN - SCOPUS:0036969043

SN - 0272-9172

VL - 724

SP - 153

EP - 158

JO - Materials Research Society Symposium - Proceedings

JF - Materials Research Society Symposium - Proceedings

T2 - Biological and Biomimetic Materials - Properties to Function

Y2 - 1 April 2002 through 5 April 2002

ER -

Colorimetric biosensor vesicles for biotechnological applications

Abstract

ASJC Scopus subject areas

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