Effect of dietary oils on the production of n-3 and n-6 metabolites of leukocyte 5-lipoxygenase in five rat strains

Leslie G. Cleland; Michael J. James; Robert A. Gibson; Joanna S. Hawkes; W. Henry Betts

doi:10.1016/0005-2760(90)90024-R

Effect of dietary oils on the production of n-3 and n-6 metabolites of leukocyte 5-lipoxygenase in five rat strains

Leslie G. Cleland
, Michael J. James
, Robert A. Gibson
, Joanna S. Hawkes
, W. Henry Betts

SAHMRI Women And Kids

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

We examined the influence of various dietary oils, including linseed and fish oil on the relative rates of leukotriene B₄ (LTB₄) and LTB₅ production by rat peritoneal exudate cells in five rat strains. While there was an association between the membrane phospholipid levels of the fatty acid precursors (arachidonic acid (AA) and eicosapentaenoic acid (EPA)) and the rate of synthesis of their respective 5-lipoxygenase products (LTB₄ and LTB₅), the rate of LTB₄ synthesis was a combined function of both AA and EPA levels. We observed a strong linear relationship (correlation coefficient = 0.99) between the ratio of EPA/AA in the cell membrane phospholipids and the ratio of LTB₅/LTB₄ produced by these cells in vitro; this association was independent of genetic (strain) variability and was independent of the source of EPA (dietary EPA or EPA endogenously synthesized from dietary α-linolenic acid).

Original language	English
Pages (from-to)	253-258
Number of pages	6
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	1043
Issue number	3
DOIs	https://doi.org/10.1016/0005-2760(90)90024-R
Publication status	Published or Issued - 17 Apr 1990

Keywords

(Rat)
5-Lipoxygenase
Fatty acid
Icosapentaenoic acid
Leukotriene
Oil

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

Access to Document

10.1016/0005-2760(90)90024-R

Cite this

@article{11a98519815e4e329b5218173da20b39,

title = "Effect of dietary oils on the production of n-3 and n-6 metabolites of leukocyte 5-lipoxygenase in five rat strains",

abstract = "We examined the influence of various dietary oils, including linseed and fish oil on the relative rates of leukotriene B4 (LTB4) and LTB5 production by rat peritoneal exudate cells in five rat strains. While there was an association between the membrane phospholipid levels of the fatty acid precursors (arachidonic acid (AA) and eicosapentaenoic acid (EPA)) and the rate of synthesis of their respective 5-lipoxygenase products (LTB4 and LTB5), the rate of LTB4 synthesis was a combined function of both AA and EPA levels. We observed a strong linear relationship (correlation coefficient = 0.99) between the ratio of EPA/AA in the cell membrane phospholipids and the ratio of LTB5/LTB4 produced by these cells in vitro; this association was independent of genetic (strain) variability and was independent of the source of EPA (dietary EPA or EPA endogenously synthesized from dietary α-linolenic acid).",

keywords = "(Rat), 5-Lipoxygenase, Fatty acid, Icosapentaenoic acid, Leukotriene, Oil",

author = "Cleland, \{Leslie G.\} and James, \{Michael J.\} and Gibson, \{Robert A.\} and Hawkes, \{Joanna S.\} and Betts, \{W. Henry\}",

note = "Funding Information: This study was supported by a grant from the National Health and Medical Research Council. M.J.J. is the recipient of an Australian Mutual Provident Society Bicentennial Career Development Award. The expert technical assistance of Cindy Hall and Geraldine Murphy is gratefully acknowledged.",

year = "1990",

month = apr,

day = "17",

doi = "10.1016/0005-2760(90)90024-R",

language = "English",

volume = "1043",

pages = "253--258",

journal = "Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism",

issn = "0005-2760",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Effect of dietary oils on the production of n-3 and n-6 metabolites of leukocyte 5-lipoxygenase in five rat strains

AU - Cleland, Leslie G.

AU - James, Michael J.

AU - Gibson, Robert A.

AU - Hawkes, Joanna S.

AU - Betts, W. Henry

N1 - Funding Information: This study was supported by a grant from the National Health and Medical Research Council. M.J.J. is the recipient of an Australian Mutual Provident Society Bicentennial Career Development Award. The expert technical assistance of Cindy Hall and Geraldine Murphy is gratefully acknowledged.

PY - 1990/4/17

Y1 - 1990/4/17

N2 - We examined the influence of various dietary oils, including linseed and fish oil on the relative rates of leukotriene B4 (LTB4) and LTB5 production by rat peritoneal exudate cells in five rat strains. While there was an association between the membrane phospholipid levels of the fatty acid precursors (arachidonic acid (AA) and eicosapentaenoic acid (EPA)) and the rate of synthesis of their respective 5-lipoxygenase products (LTB4 and LTB5), the rate of LTB4 synthesis was a combined function of both AA and EPA levels. We observed a strong linear relationship (correlation coefficient = 0.99) between the ratio of EPA/AA in the cell membrane phospholipids and the ratio of LTB5/LTB4 produced by these cells in vitro; this association was independent of genetic (strain) variability and was independent of the source of EPA (dietary EPA or EPA endogenously synthesized from dietary α-linolenic acid).

AB - We examined the influence of various dietary oils, including linseed and fish oil on the relative rates of leukotriene B4 (LTB4) and LTB5 production by rat peritoneal exudate cells in five rat strains. While there was an association between the membrane phospholipid levels of the fatty acid precursors (arachidonic acid (AA) and eicosapentaenoic acid (EPA)) and the rate of synthesis of their respective 5-lipoxygenase products (LTB4 and LTB5), the rate of LTB4 synthesis was a combined function of both AA and EPA levels. We observed a strong linear relationship (correlation coefficient = 0.99) between the ratio of EPA/AA in the cell membrane phospholipids and the ratio of LTB5/LTB4 produced by these cells in vitro; this association was independent of genetic (strain) variability and was independent of the source of EPA (dietary EPA or EPA endogenously synthesized from dietary α-linolenic acid).

KW - (Rat)

KW - 5-Lipoxygenase

KW - Fatty acid

KW - Icosapentaenoic acid

KW - Leukotriene

KW - Oil

UR - https://www.scopus.com/pages/publications/0025276456

U2 - 10.1016/0005-2760(90)90024-R

DO - 10.1016/0005-2760(90)90024-R

M3 - Article

C2 - 2157490

AN - SCOPUS:0025276456

SN - 0005-2760

VL - 1043

SP - 253

EP - 258

JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

IS - 3

ER -

Effect of dietary oils on the production of n-3 and n-6 metabolites of leukocyte 5-lipoxygenase in five rat strains

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this