Effect of Long-term Exposure to Peptides on Mono- and Multispecies Biofilms in Dentinal Tubules

Xiangya Huang, Markus Haapasalo, Zhejun Wang, Robert E.W. Hancock, Xi Wei, Ya Shen

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Introduction: The aim of this study was to evaluate the antibiofilm effectiveness of 2% chlorhexidine (CHX) and peptides 1018 and DJK-5 used either alone or in a mixture (peptide and 2% CHX) against Enterococcus faecalis and multispecies biofilms in dentin canals after short-term and long-term exposure. Methods: One hundred eighty dentin blocks were prepared and filled with E. faecalis or multispecies bacteria by centrifugation. Three-week-old biofilms in dentin were subjected to 2% CHX, DJK-5 (10 μg/mL), 1018 (10 μg/mL), DJK-5 + 2% CHX, or 1018 + 2% CHX for short-term (1 or 3 minutes), short-term exposure after 24 hours, and long-term exposure (24 hours of exposure). The antibacterial efficacy was determined by live/dead bacterial viability staining and confocal laser scanning microscopy. Results: Peptide DJK-5 with or without CHX was the most effective agent against all the biofilms (P < .05), killing 77% of biofilm bacteria in 1 minute. No significant difference in bacterial killing was detected between the first 3 minutes of exposure (>81%) and after 24 hours of exposure (83%) to DJK-5 or DJK-5 + CHX. Chlorhexidine and peptide 1018 had a weaker antibiofilm effect than DJK-5, and their effect was time dependent (P < .05) with a maximum killing of 60% after 24 hours of exposure. Conclusions: Peptide DJK-5 alone and together with CHX had a rapid antibacterial effect against dentin infection. An additional antibacterial effect by CHX and peptide 1018 was achieved after a 24-hour long-term exposure.

Original languageEnglish
Pages (from-to)1522-1528
Number of pages7
JournalJournal of Endodontics
Issue number12
Publication statusPublished or Issued - Dec 2019
Externally publishedYes


  • Biofilms
  • chlorhexidine
  • long-term exposure
  • peptides
  • short-term exposure

ASJC Scopus subject areas

  • Dentistry(all)

Cite this