Efficacy of a synthetic calcium phosphate with submicron surface topography as autograft extender in lapine posterolateral spinal fusion

Lukas A. van Dijk, Davide Barbieri, Florence Barrère-de Groot, Huipin Yuan, Rema Oliver, Chris Christou, William R. Walsh, Joost D. de Bruijn

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11 Citations (Scopus)


Posterolateral spinal fusion (PLF) is a common procedure in orthopedic surgery that is performed to fuse adjacent vertebrae to reduce symptoms related to spinal conditions. In the current study, a novel synthetic calcium phosphate with submicron surface topography was evaluated as an autograft extender in a validated rabbit model of PLF. Fifty-nine skeletally mature New Zealand white rabbits were divided into three groups and underwent single-level intertransverse process PLF at L4-5 using (1) autologous bone graft (ABG) alone or in a 1:1 combination with (2) calcium phosphate granules (ABG/BCPgranules), or (3) granules embedded in a fast-resorbing polymeric carrier (ABG/BCPputty). After 6, 9, and 12 weeks, animals were sacrificed and spinal fusion was assessed by manual palpation, Radiographs, micro-CT, mechanical testing (12 weeks only), histology, and histomorphometry. Based on all endpoints, all groups showed a gradual progression in bone formation and maturation during time, leading to solid fusion masses between the transverse processes after 12 weeks. Fusion assessments by manual palpation, radiography and histology were consistent and demonstrated equivalent fusion rates between groups, with high bilateral fusion rates after 12 weeks. Mechanical tests after 12 weeks indicated substantially lower range of motion for all groups, compared to non-operated controls. By histology and histomorphometry, the gradual formation and maturation of bone in the fusion mass was confirmed for each graft type. With these results, we describe the equivalent performance between autograft and a novel calcium phosphate material as an autograft extender in a rabbit model of PLF using an extensive range of evaluation techniques.

Original languageEnglish
Pages (from-to)2080-2090
Number of pages11
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number6
Publication statusPublished or Issued - Aug 2019
Externally publishedYes


  • calcium phosphate
  • posterolateral spinal fusion
  • rabbit
  • submicron surface topography

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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