Ethanol extract of achillea fragrantissima enhances angiogenesis through stimulation of vegf production

Hana M. Hammad, Amer Imraish, Maysa Al-Hussaini, Malek Zihlif, Amani A. Harb, Tuqa M. Abu Thiab, Zainab Lafi, Zeyad D. Nassar, Fatma U. Afifi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using a rat aortic ring assay and in vivo using a rat excision wound model. Results: In concentrations of 50 and 100 μg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulated the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

Original languageEnglish
Pages (from-to)2035-2042
Number of pages8
JournalEndocrine, Metabolic and Immune Disorders - Drug Targets
Issue number11
Publication statusPublished or Issued - Nov 2021


  • Achillea fragrantissima L
  • Angiogenesis
  • Aortic ring assay
  • Asteraceae
  • VEGF
  • Wound healing

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Immunology and Allergy

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