Expression of the costimulator molecules, CD80, CD86, CD28, and CD152 on lymphocytes from neonates and young children

Salenna R. Elliott, Peter J. Macardle, Don M. Roberton, Heddy Zola

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    17 Citations (Scopus)

    Abstract

    The expression of CD80, CD86, CD28, and CD152 were examined on peripheral blood lymphocytes from adults, neonates (cord blood lymphocytes) and young children (2-20 months of age). There was no difference in the expression of CD80 or CD86 between adult and neonatal B cells, either resting or activated. A higher percentage of resting T cells expressed CD28 in neonates and young children compared to adults. CD28 expression was similar on adult and neonatal T cells activated with PMA and ionomycin. However, CD28 was expressed at greater intensity on a higher percentage of neonatal T cells than adult T cells stimulated with CD3. CD152 expression was lower on neonatal T cells than adult T cells stimulated with PMA and ionomycin and undetectable on neonatal T cells stimulated with CD3. In contrast, intracellular CD152 was equivalent in adult and neonatal T cells stimulated with PMA and ionomycin, suggesting trafficking of CD152 to the cell surface may be differentially regulated in neonatal T cells. Since the T cell response is determined by the balance of signals received from CD28 and CD152, high levels of CD28 expression and lower surface expression of CD152 on neonatal T cells may represent specialisation to promote activation of neonatal T cells.

    Original languageEnglish
    Pages (from-to)1039-1048
    Number of pages10
    JournalHuman Immunology
    Volume60
    Issue number11
    DOIs
    Publication statusPublished or Issued - Nov 1999

    Keywords

    • B lymphocytes
    • Costimulator molecules
    • Neonate
    • T lymphocytes

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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