Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein

Elizabeth A. Worobec; Richard J. Siehnel; Paul Gladman; Robert E.W. Hancock

doi:10.1111/j.1574-6968.1988.tb02602.x

Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein

Elizabeth A. Worobec
, Richard J. Siehnel
, Paul Gladman
, Robert E.W. Hancock

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcloning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was transferred to P. aeruginosa pstS mutants. The P. aeruoginosa phosphate-binding protein was also expressed and secreted into the periplasm of E. coli pstS mutants.

Original language	English
Pages (from-to)	235-238
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	52
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1988.tb02602.x
Publication status	Published or Issued - Aug 1988
Externally published	Yes

Keywords

Phosphate-binding protein
Pseudomonas aeruginosa
pstS gene cloning

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1988.tb02602.x

Cite this

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title = "Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein",

abstract = "The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcloning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was transferred to P. aeruginosa pstS mutants. The P. aeruoginosa phosphate-binding protein was also expressed and secreted into the periplasm of E. coli pstS mutants.",

keywords = "Phosphate-binding protein, Pseudomonas aeruginosa, pstS gene cloning",

author = "Worobec, \{Elizabeth A.\} and Siehnel, \{Richard J.\} and Paul Gladman and Hancock, \{Robert E.W.\}",

note = "Funding Information: This work was financially supported by a grant from the Natural Science and Engineering Research Council of Canada to R.E.W.H.E.A.W. was a fellow of the Alberta Heritage Foundation for Medical Research.",

year = "1988",

month = aug,

doi = "10.1111/j.1574-6968.1988.tb02602.x",

language = "English",

volume = "52",

pages = "235--238",

journal = "FEMS Microbiology Letters",

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publisher = "Oxford University Press",

number = "3",

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TY - JOUR

T1 - Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein

AU - Worobec, Elizabeth A.

AU - Siehnel, Richard J.

AU - Gladman, Paul

AU - Hancock, Robert E.W.

N1 - Funding Information: This work was financially supported by a grant from the Natural Science and Engineering Research Council of Canada to R.E.W.H.E.A.W. was a fellow of the Alberta Heritage Foundation for Medical Research.

PY - 1988/8

Y1 - 1988/8

N2 - The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcloning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was transferred to P. aeruginosa pstS mutants. The P. aeruoginosa phosphate-binding protein was also expressed and secreted into the periplasm of E. coli pstS mutants.

AB - The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcloning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was transferred to P. aeruginosa pstS mutants. The P. aeruoginosa phosphate-binding protein was also expressed and secreted into the periplasm of E. coli pstS mutants.

KW - Phosphate-binding protein

KW - Pseudomonas aeruginosa

KW - pstS gene cloning

UR - https://www.scopus.com/pages/publications/0023774276

U2 - 10.1111/j.1574-6968.1988.tb02602.x

DO - 10.1111/j.1574-6968.1988.tb02602.x

M3 - Article

AN - SCOPUS:0023774276

SN - 0378-1097

VL - 52

SP - 235

EP - 238

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 3

ER -

Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein

Abstract

Keywords

ASJC Scopus subject areas

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