TY - JOUR
T1 - Gene cloning and expression of the Pseudomonas aeruginosa periplasmic phosphate-binding protein
AU - Worobec, Elizabeth A.
AU - Siehnel, Richard J.
AU - Gladman, Paul
AU - Hancock, Robert E.W.
N1 - Funding Information:
This work was financially supported by a grant from the Natural Science and Engineering Research Council of Canada to R.E.W.H.E.A.W. was a fellow of the Alberta Heritage Foundation for Medical Research.
PY - 1988/8
Y1 - 1988/8
N2 - The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcloning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was transferred to P. aeruginosa pstS mutants. The P. aeruoginosa phosphate-binding protein was also expressed and secreted into the periplasm of E. coli pstS mutants.
AB - The pstS gene, encoding the Pseudomonas aeruginosa phosphate-binding protein, was cloned onto a cosmid vector into Escherichia coli, and localized by subcloning, mapping the insertion site of Tn501 in a P. aeruginosa pstS::Tn501 mutant, and hybridization to an oligonucleotide pool synthesized according to the aminoterminal amino acid sequence of the purified protein. The cloned pstS gene was transferred to P. aeruginosa pstS mutants. The P. aeruoginosa phosphate-binding protein was also expressed and secreted into the periplasm of E. coli pstS mutants.
KW - Phosphate-binding protein
KW - Pseudomonas aeruginosa
KW - pstS gene cloning
UR - https://www.scopus.com/pages/publications/0023774276
U2 - 10.1111/j.1574-6968.1988.tb02602.x
DO - 10.1111/j.1574-6968.1988.tb02602.x
M3 - Article
AN - SCOPUS:0023774276
SN - 0378-1097
VL - 52
SP - 235
EP - 238
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
ER -