TY - JOUR
T1 - Genetic and functional analysis of R5X4 human immunodeficiency virus type 1 envelope glycoproteins derived from two individuals homozygous for the CCR5Δ32 allele
AU - Gray, Lachlan
AU - Churchill, Melissa J.
AU - Keane, Niamh
AU - Sterjovski, Jasminka
AU - Ellett, Anne M.
AU - Purcell, Damian F.J.
AU - Poumbourios, Pantelis
AU - Kol, Chenda
AU - Wang, Bin
AU - Saksena, Nitin K.
AU - Wesselingh, Steven L.
AU - Price, Patricia
AU - French, Martyn
AU - Gabuzda, Dana
AU - Gorry, Paul R.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/4
Y1 - 2006/4
N2 - We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5Δ32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral blood mononuclear cells (PBMC), but a subset was dually lymphocyte- and macrophage-tropic and used either CCR5 or CXCR4 for entry into PBMC and monocyte-derived macrophages. The persistence of CCR5- using HIV-1 in two CCR5Δ32 homozygotes suggests the conserved CCR5 binding domain of Env is highly stable and provides new mechanistic insights important for HIV-1 transmission and persistence.
AB - We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5Δ32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral blood mononuclear cells (PBMC), but a subset was dually lymphocyte- and macrophage-tropic and used either CCR5 or CXCR4 for entry into PBMC and monocyte-derived macrophages. The persistence of CCR5- using HIV-1 in two CCR5Δ32 homozygotes suggests the conserved CCR5 binding domain of Env is highly stable and provides new mechanistic insights important for HIV-1 transmission and persistence.
UR - http://www.scopus.com/inward/record.url?scp=33645217128&partnerID=8YFLogxK
U2 - 10.1128/JVI.80.7.3684-3691.2006
DO - 10.1128/JVI.80.7.3684-3691.2006
M3 - Article
C2 - 16537640
AN - SCOPUS:33645217128
SN - 0022-538X
VL - 80
SP - 3684
EP - 3691
JO - Journal of Virology
JF - Journal of Virology
IS - 7
ER -