Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models

Stefan P W de Vries; Srishti Gupta; Abiyad Baig; Elli Wright; Amy Wedley; Annette Nygaard Jensen; Lizeth LaCharme Lora; Suzanne Humphrey; Henrik Skovgård; Kareen Macleod; Elsa Pont; Dominika P Wolanska; Joanna L'Heureux; Fredrick M Mobegi; David G E Smith; Paul Everest; Aldert Zomer; Nicola Williams; Paul Wigley; Thomas Humphrey; Duncan J Maskell; Andrew J Grant

doi:10.1038/s41598-017-01133-4

Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models

Stefan P W de Vries, Srishti Gupta, Abiyad Baig, Elli Wright, Amy Wedley, Annette Nygaard Jensen, Lizeth LaCharme Lora, Suzanne Humphrey, Henrik Skovgård, Kareen Macleod, Elsa Pont, Dominika P Wolanska, Joanna L'Heureux, Fredrick M Mobegi, David G E Smith, Paul Everest, Aldert Zomer, Nicola Williams, Paul Wigley, Thomas HumphreyDuncan J Maskell, Andrew J Grant

Microbiome And Host Health

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and -poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.

Original language	English
Pages (from-to)	1251
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-01133-4
Publication status	Published or Issued - 28 Apr 2017

Keywords

Journal Article

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10.1038/s41598-017-01133-4

Cite this

de Vries, S. P. W., Gupta, S., Baig, A., Wright, E., Wedley, A., Jensen, A. N., Lora, L. L., Humphrey, S., Skovgård, H., Macleod, K., Pont, E., Wolanska, D. P., L'Heureux, J., Mobegi, F. M., Smith, D. G. E., Everest, P., Zomer, A., Williams, N., Wigley, P., ... Grant, A. J. (2017). Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models. Scientific Reports, 7(1), 1251. https://doi.org/10.1038/s41598-017-01133-4

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de Vries, SPW, Gupta, S, Baig, A, Wright, E, Wedley, A, Jensen, AN, Lora, LL, Humphrey, S, Skovgård, H, Macleod, K, Pont, E, Wolanska, DP, L'Heureux, J, Mobegi, FM, Smith, DGE, Everest, P, Zomer, A, Williams, N, Wigley, P, Humphrey, T, Maskell, DJ & Grant, AJ 2017, 'Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models', Scientific Reports, vol. 7, no. 1, pp. 1251. https://doi.org/10.1038/s41598-017-01133-4

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T1 - Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models

AU - de Vries, Stefan P W

AU - Gupta, Srishti

AU - Baig, Abiyad

AU - Wright, Elli

AU - Wedley, Amy

AU - Jensen, Annette Nygaard

AU - Lora, Lizeth LaCharme

AU - Humphrey, Suzanne

AU - Skovgård, Henrik

AU - Macleod, Kareen

AU - Pont, Elsa

AU - Wolanska, Dominika P

AU - L'Heureux, Joanna

AU - Mobegi, Fredrick M

AU - Smith, David G E

AU - Everest, Paul

AU - Zomer, Aldert

AU - Williams, Nicola

AU - Wigley, Paul

AU - Humphrey, Thomas

AU - Maskell, Duncan J

AU - Grant, Andrew J

PY - 2017/4/28

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N2 - Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and -poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.

AB - Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and -poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.

KW - Journal Article

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DO - 10.1038/s41598-017-01133-4

M3 - Article

C2 - 28455506

SN - 2045-2322

VL - 7

SP - 1251

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -