Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B

Nigel T. Price; Giulio Francia; Len Hall; Christopher G. Proud

doi:10.1016/0167-4781(94)90037-X

Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B

Nigel T. Price, Giulio Francia, Len Hall, Christopher G. Proud

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product.

Original language	English
Pages (from-to)	207-210
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1217
Issue number	2
DOIs	https://doi.org/10.1016/0167-4781(94)90037-X
Publication status	Published or Issued - 1 Mar 1994
Externally published	Yes

Keywords

Eukaryote
Guanine nucleotide exchange factor
Initiation factor-2B
Protein synthesis

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(94)90037-X

Cite this

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title = "Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B",

abstract = "Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product.",

keywords = "Eukaryote, Guanine nucleotide exchange factor, Initiation factor-2B, Protein synthesis",

author = "Price, {Nigel T.} and Giulio Francia and Len Hall and Proud, {Christopher G.}",

note = "Funding Information: This work was supported by grants from the Medical Research Council (UK) and the Wellcome Trust. Giulio Francia was supported by a grant from the Nuffield Foundation. The authors would like to thank Helen Downer for performing automated DNA sequencing. The rabbit reticulocyte cDNA library was kindly provided by Dr. J.-J. Chen, M.I.T., MA, USA.",

year = "1994",

month = mar,

day = "1",

doi = "10.1016/0167-4781(94)90037-X",

language = "English",

volume = "1217",

pages = "207--210",

journal = "BBA - Gene Structure and Expression",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B

AU - Price, Nigel T.

AU - Francia, Giulio

AU - Hall, Len

AU - Proud, Christopher G.

N1 - Funding Information: This work was supported by grants from the Medical Research Council (UK) and the Wellcome Trust. Giulio Francia was supported by a grant from the Nuffield Foundation. The authors would like to thank Helen Downer for performing automated DNA sequencing. The rabbit reticulocyte cDNA library was kindly provided by Dr. J.-J. Chen, M.I.T., MA, USA.

PY - 1994/3/1

Y1 - 1994/3/1

N2 - Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product.

AB - Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product.

KW - Eukaryote

KW - Guanine nucleotide exchange factor

KW - Initiation factor-2B

KW - Protein synthesis

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U2 - 10.1016/0167-4781(94)90037-X

DO - 10.1016/0167-4781(94)90037-X

M3 - Article

C2 - 8110836

AN - SCOPUS:0028203751

SN - 0167-4781

VL - 1217

SP - 207

EP - 210

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 2

ER -

Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B

Abstract

Keywords

ASJC Scopus subject areas

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