Guanine nucleotide exchange factor for eukaryotic initiation factor-2. Cloning of cDNA for the δ-subunit of rabbit translation initiation factor-2B

Nigel T. Price, Giulio Francia, Len Hall, Christopher G. Proud

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15 Citations (Scopus)


Peptide sequence data for rabbit eIF-2Bδ were obtained and used to design redundant oligonucleotides for PCR. RNA was isolated from rabbit liver and used to direct the synthesis of total cDNA. A rabbit eIF-2Bδ transcript was then amplified by PCR and sequenced. The PCR product was used to isolate a clone from a rabbit liver cDNA library. RACE (rapid amplification of cDNA ends) was used to obtain further 5′ sequence. Subsequently, a full length cDNA was obtained from a rabbit reticulocyte library. PCR was used to confirm that the sequence is the same for the liver factor. The sequence obtained shows strong homology to that of yeast eIF-2Bδ, the GCD2 gene product.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalBBA - Gene Structure and Expression
Issue number2
Publication statusPublished or Issued - 1 Mar 1994
Externally publishedYes


  • Eukaryote
  • Guanine nucleotide exchange factor
  • Initiation factor-2B
  • Protein synthesis

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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