Gut microbiota transplantation for colonization of germ-free mice

Jocelyn M. Choo; Geraint B. Rogers

doi:10.1016/j.xpro.2021.100610

Gut microbiota transplantation for colonization of germ-free mice

Jocelyn M. Choo, Geraint B. Rogers

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery. For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021).

Original language	English
Article number	100610
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100610
Publication status	Published or Issued - 17 Sept 2021

Keywords

Microbiology
Model Organisms
Molecular Biology
Sequence analysis

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience

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10.1016/j.xpro.2021.100610

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abstract = "The use of germ-free mice is integral to the understanding of host-gut microbiome relationships. Such models rely on faithful replication of the donor microbiome to establish causal effects of the gut microbiota on host pathophysiology. This protocol describes the preparation and transfer of donor microbiota, focusing on strict anaerobic processing methods and multiple instillations by gavage for optimal gut microbiota recovery. For complete details on the generation and use of this protocol, please refer to Choo and Rogers (2021).",

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KW - Microbiology

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KW - Molecular Biology

KW - Sequence analysis

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Gut microbiota transplantation for colonization of germ-free mice

Abstract

Keywords

ASJC Scopus subject areas

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