Human astrocytes express membrane cofactor protein (CD46), a regulator of complement activation

David L. Gordon; Tania A. Sadlon; Steve L. Wesselingh; Sarah M. Russell; Ricky W. Johnstone; Damian F.J. Purcell

doi:10.1016/0165-5728(92)90051-L

Human astrocytes express membrane cofactor protein (CD46), a regulator of complement activation

David L. Gordon, Tania A. Sadlon, Steve L. Wesselingh, Sarah M. Russell, Ricky W. Johnstone, Damian F.J. Purcell

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Expression of membrane cofactor protein (CD46) on cultured human astrocytes was demonstrated by indirect immunofluorescence microscopy and flow cytometry following staining with a monoclonal antibody specific for CD46. Western transfer and immunoblotting detected a doublet of M_r 66,000 and 56,000. Analysis of astrocyte mRNA revealed the presence of multiple alternatively spliced transcripts encoding different extracellular regions or cytoplasmic tails of CD46. Astrocytes were also shown to express decay accelerating factor, but not the type 1 complement receptor. Upregulation of astrocyte CD46 occurred following cytomegalovirus infection. These results indicate that astrocytes express proteins involved in regulation of complement activation and protection against autologous complement.

Original language	English
Pages (from-to)	199-208
Number of pages	10
Journal	Journal of Neuroimmunology
Volume	36
Issue number	2-3
DOIs	https://doi.org/10.1016/0165-5728(92)90051-L
Publication status	Published or Issued - Feb 1992
Externally published	Yes

Keywords

Astrocyte
Complement
Membrane cofactor protein
Regulator of complement activation

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/0165-5728(92)90051-L

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title = "Human astrocytes express membrane cofactor protein (CD46), a regulator of complement activation",

abstract = "Expression of membrane cofactor protein (CD46) on cultured human astrocytes was demonstrated by indirect immunofluorescence microscopy and flow cytometry following staining with a monoclonal antibody specific for CD46. Western transfer and immunoblotting detected a doublet of Mr 66,000 and 56,000. Analysis of astrocyte mRNA revealed the presence of multiple alternatively spliced transcripts encoding different extracellular regions or cytoplasmic tails of CD46. Astrocytes were also shown to express decay accelerating factor, but not the type 1 complement receptor. Upregulation of astrocyte CD46 occurred following cytomegalovirus infection. These results indicate that astrocytes express proteins involved in regulation of complement activation and protection against autologous complement.",

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author = "Gordon, {David L.} and Sadlon, {Tania A.} and Wesselingh, {Steve L.} and Russell, {Sarah M.} and Johnstone, {Ricky W.} and Purcell, {Damian F.J.}",

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T1 - Human astrocytes express membrane cofactor protein (CD46), a regulator of complement activation

AU - Gordon, David L.

AU - Sadlon, Tania A.

AU - Wesselingh, Steve L.

AU - Russell, Sarah M.

AU - Johnstone, Ricky W.

AU - Purcell, Damian F.J.

PY - 1992/2

Y1 - 1992/2

N2 - Expression of membrane cofactor protein (CD46) on cultured human astrocytes was demonstrated by indirect immunofluorescence microscopy and flow cytometry following staining with a monoclonal antibody specific for CD46. Western transfer and immunoblotting detected a doublet of Mr 66,000 and 56,000. Analysis of astrocyte mRNA revealed the presence of multiple alternatively spliced transcripts encoding different extracellular regions or cytoplasmic tails of CD46. Astrocytes were also shown to express decay accelerating factor, but not the type 1 complement receptor. Upregulation of astrocyte CD46 occurred following cytomegalovirus infection. These results indicate that astrocytes express proteins involved in regulation of complement activation and protection against autologous complement.

AB - Expression of membrane cofactor protein (CD46) on cultured human astrocytes was demonstrated by indirect immunofluorescence microscopy and flow cytometry following staining with a monoclonal antibody specific for CD46. Western transfer and immunoblotting detected a doublet of Mr 66,000 and 56,000. Analysis of astrocyte mRNA revealed the presence of multiple alternatively spliced transcripts encoding different extracellular regions or cytoplasmic tails of CD46. Astrocytes were also shown to express decay accelerating factor, but not the type 1 complement receptor. Upregulation of astrocyte CD46 occurred following cytomegalovirus infection. These results indicate that astrocytes express proteins involved in regulation of complement activation and protection against autologous complement.

KW - Astrocyte

KW - Complement

KW - Membrane cofactor protein

KW - Regulator of complement activation

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Human astrocytes express membrane cofactor protein (CD46), a regulator of complement activation

Abstract

Keywords

ASJC Scopus subject areas

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