Hyaluronic acid synthesis in a cell-free system from rat fibrosarcoma

J. J. Hopwood, F. W. Fitch, A. Dorfman

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A transplantable rat fibrosarcoma (in both ascites and solid forms) has been shown to contain and synthesize large amounts of hyaluronic acid. A particulate hyaluronic acid synthesizing system has been isolated from the solid fibrosarcoma and some characteristics of the enzyme system are detailed. The enzyme complex transferred GlcUA or GlcNAc from UDP-GlcUA or UDP-GlcNAc at a rate of approximately 60 nmole/hr/mg protein to extend hyaluronic acid chains by approximately 45,000 daltons.

Original languageEnglish
Pages (from-to)583-590
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished or Issued - 27 Nov 1974

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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