Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase

Sébastien Pyr Dit Ruys, Xuemin Wang, Ewan M. Smith, Gaëtan Herinckx, Nusrat Hussain, Mark H. Rider, Didier Vertommen, Christopher G. Proud

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49 Citations (Scopus)

Abstract

eEF2K [eEF2 (eukaryotic elongation factor 2) kinase] phosphorylates and inactivates the translation elongation factor eEF2. eEF2K is not a member of the main eukaryotic protein kinase superfamily, but instead belongs to a small group of so-called α-kinases. The activity of eEF2K is normally dependent upon Ca 2+ and calmodulin. eEF2K has previously been shown to undergo autophosphorylation, the stoichiometry of which suggested the existence of multiple sites. In the present study we have identified several autophosphorylation sites, including Thr 348, Thr 353, Ser 366 and Ser 445, all of which are highly conserved among vertebrate eEF2Ks. We also identified a number of other sites, including Ser 78, a known site of phosphorylation, and others, some of which are less well conserved. None of the sites lies in the catalytic domain, but three affect eEF2K activity. Mutation of Ser 78, Thr 348 and Ser 366 to a non-phosphorylatable alanine residue decreased eEF2K activity. Phosphorylation of Thr 348 was detected by immunoblotting after transfecting wild-type eEF2K into HEK (human embryonic kidney)-293 cells, but not after transfection with a kinase-inactive construct, confirming that this is indeed a site of autophosphorylation. Thr 348 appears to be constitutively autophosphorylated in vitro. Interestingly, other recent data suggest that the corresponding residue in other α-kinases is also autophosphorylated and contributes to the activation of these enzymes [Crawley, Gharaei, Ye, Yang, Raveh, London, Schueler-Furman, Jia and Cote (2011) J. Biol. Chem. 286, 2607-2616]. Ser 366 phosphorylation was also detected in intact cells, but was still observed in the kinase-inactive construct, demonstrating that this site is phosphorylated not only autocatalytically but also in trans by other kinases.

Original languageEnglish
Pages (from-to)681-692
Number of pages12
JournalBiochemical Journal
Volume442
Issue number3
DOIs
Publication statusPublished or Issued - 15 Mar 2012
Externally publishedYes

Keywords

  • Calmodulin
  • Elongation
  • Eukaryotic elongation factor 2 (eEF2)
  • Mass spectrometry (MS)
  • Translation
  • α-kinase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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