Imaging blood vessels in the zebrafish

Makoto Kamei, Sumio Isogai, Weijun Pan, Brant M. Weinstein

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.

Original languageEnglish
Pages (from-to)27-54
Number of pages28
JournalMethods in Cell Biology
Volume100
Issue numberC
DOIs
Publication statusPublished or Issued - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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