Imaging blood vessels in the zebrafish

Makoto Kamei; Sumio Isogai; Weijun Pan; Brant M. Weinstein

doi:10.1016/B978-0-12-384892-5.00002-5

Imaging blood vessels in the zebrafish

Makoto Kamei, Sumio Isogai, Weijun Pan, Brant M. Weinstein

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.

Original language	English
Pages (from-to)	27-54
Number of pages	28
Journal	Methods in Cell Biology
Volume	100
Issue number	C
DOIs	https://doi.org/10.1016/B978-0-12-384892-5.00002-5
Publication status	Published or Issued - 2010
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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10.1016/B978-0-12-384892-5.00002-5

Cite this

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AU - Isogai, Sumio

AU - Pan, Weijun

AU - Weinstein, Brant M.

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AB - Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.

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JF - Methods in Cell Biology

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Imaging blood vessels in the zebrafish

Abstract

ASJC Scopus subject areas

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