Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

T. W.V. Daniels; G. B. Rogers; F. A. Stressmann; C. J. van der Gast; K. D. Bruce; G. R. Jones; G. J. Connett; J. P. Legg; M. P. Carroll

doi:10.1016/j.jcf.2012.05.008

Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

T. W.V. Daniels, G. B. Rogers, F. A. Stressmann, C. J. van der Gast, K. D. Bruce, G. R. Jones, G. J. Connett, J. P. Legg, M. P. Carroll

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48 Citations (Scopus)

Abstract

Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F_1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F_1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.

Original language	English
Pages (from-to)	22-28
Number of pages	7
Journal	Journal of Cystic Fibrosis
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/j.jcf.2012.05.008
Publication status	Published or Issued - Jan 2013
Externally published	Yes

Keywords

Antibiotics
Bacterial diversity
Pulmonary exacerbation
Relative abundance
T-RFLP

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Pulmonary and Respiratory Medicine

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10.1016/j.jcf.2012.05.008

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title = "Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis",

abstract = "Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.",

keywords = "Antibiotics, Bacterial diversity, Pulmonary exacerbation, Relative abundance, T-RFLP",

author = "Daniels, {T. W.V.} and Rogers, {G. B.} and Stressmann, {F. A.} and {van der Gast}, {C. J.} and Bruce, {K. D.} and Jones, {G. R.} and Connett, {G. J.} and Legg, {J. P.} and Carroll, {M. P.}",

note = "Funding Information: This work was supported by The Anna Trust . ",

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doi = "10.1016/j.jcf.2012.05.008",

language = "English",

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T1 - Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

AU - Daniels, T. W.V.

AU - Rogers, G. B.

AU - Stressmann, F. A.

AU - van der Gast, C. J.

AU - Bruce, K. D.

AU - Jones, G. R.

AU - Connett, G. J.

AU - Legg, J. P.

AU - Carroll, M. P.

N1 - Funding Information: This work was supported by The Anna Trust .

PY - 2013/1

Y1 - 2013/1

N2 - Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.

AB - Background: A diverse array of bacterial species is present in the CF airways, in addition to those recognised as clinically important. Here, we investigated the relative impact of antibiotics, used predominantly to target Pseudomonas aeruginosa during acute exacerbations, on other non-pseudomonal species. Methods: The relative abundance of viable P. aeruginosa and non-pseudomonal species was determined in sputa from 12 adult CF subjects 21, 14, and 7. days prior to antibiotics, day 3 of treatment, the final day of treatment, and 10-14. days afterwards, by T-RFLP profiling. Results: Overall, relative P. aeruginosa abundance increased during antibiotic therapy compared to other bacterial species; mean abundance pre-antibiotic 51.0±36.0% increasing to 71.3±30.4% during antibiotic (ANOVA: F1,54=5.16; P<0.027). Further, the number of non-pseudomonal species detected fell; pre-antibiotic 6.0±3.3 decreasing to 3.7±3.3 during treatment (ANOVA: F1,66=5.11; P<0.027). Conclusions: Antibiotic treatment directed at P. aeruginosa has an additional significant impact on non-pseudomonal, co-colonising species.

KW - Antibiotics

KW - Bacterial diversity

KW - Pulmonary exacerbation

KW - Relative abundance

KW - T-RFLP

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Impact of antibiotic treatment for pulmonary exacerbations on bacterial diversity in cystic fibrosis

Abstract

Keywords

ASJC Scopus subject areas

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