In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon

U. H. Stroeher; L. E. Karageorgos; R. Morona; P. A. Manning

doi:10.1016/0378-1119(94)00923-G

In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon

U. H. Stroeher, L. E. Karageorgos, R. Morona, P. A. Manning

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The rfaD gene of Escherichia coli encodes ADP-L-glycero-D-mannoheptose-6-epimerase, an enzyme required for the biosynthesis of the lipopolysaccharide (LPS) precursor ADP-L-glycero-v-mannoheptose, associated with production of the core oligosaccharide. We have identified an rfaD homologue in Vibrio cholerae 01. This gene maps adjacent to the rfb region encoding O-antigen biosynthesis, but is transcribed divergently. The complete nucleotide sequence of rfaD and the flanking DNA has been determined, and rfaD would appear to be the only gene homologous to known LPS core biosynthesis genes in this region. Comparison with the E. coli rfaD shows many similar structural features such as the ADP-binding βαβ fold at the N terminus, as well as a high degree of homology of both the nucleotide and amino-acid sequences. Based on homology, rfaD of V. cholerae may be transcribed using both σ ⁷⁰-and σ ⁵⁴-dependent promoters.

Original language	English
Pages (from-to)	67-72
Number of pages	6
Journal	Gene
Volume	155
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(94)00923-G
Publication status	Published or Issued - 21 Mar 1995
Externally published	Yes

Keywords

ADP-binding fold
Lipopolysaccharide
core oligosaccharide
heptose biosynthesis

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(94)00923-G

Cite this

@article{3039d01eeb5f42f1aff1c19e8a0e4138,

title = "In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon",

abstract = "The rfaD gene of Escherichia coli encodes ADP-L-glycero-D-mannoheptose-6-epimerase, an enzyme required for the biosynthesis of the lipopolysaccharide (LPS) precursor ADP-L-glycero-v-mannoheptose, associated with production of the core oligosaccharide. We have identified an rfaD homologue in Vibrio cholerae 01. This gene maps adjacent to the rfb region encoding O-antigen biosynthesis, but is transcribed divergently. The complete nucleotide sequence of rfaD and the flanking DNA has been determined, and rfaD would appear to be the only gene homologous to known LPS core biosynthesis genes in this region. Comparison with the E. coli rfaD shows many similar structural features such as the ADP-binding βαβ fold at the N terminus, as well as a high degree of homology of both the nucleotide and amino-acid sequences. Based on homology, rfaD of V. cholerae may be transcribed using both σ 70-and σ 54-dependent promoters.",

keywords = "ADP-binding fold, Lipopolysaccharide, core oligosaccharide, heptose biosynthesis",

author = "Stroeher, \{U. H.\} and Karageorgos, \{L. E.\} and R. Morona and Manning, \{P. A.\}",

note = "Funding Information: The authors wish to thank the Australian Research Council and the Clive and Vera Ramaciotti Foundations for financial support.",

year = "1995",

month = mar,

day = "21",

doi = "10.1016/0378-1119(94)00923-G",

language = "English",

volume = "155",

pages = "67--72",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon

AU - Stroeher, U. H.

AU - Karageorgos, L. E.

AU - Morona, R.

AU - Manning, P. A.

N1 - Funding Information: The authors wish to thank the Australian Research Council and the Clive and Vera Ramaciotti Foundations for financial support.

PY - 1995/3/21

Y1 - 1995/3/21

N2 - The rfaD gene of Escherichia coli encodes ADP-L-glycero-D-mannoheptose-6-epimerase, an enzyme required for the biosynthesis of the lipopolysaccharide (LPS) precursor ADP-L-glycero-v-mannoheptose, associated with production of the core oligosaccharide. We have identified an rfaD homologue in Vibrio cholerae 01. This gene maps adjacent to the rfb region encoding O-antigen biosynthesis, but is transcribed divergently. The complete nucleotide sequence of rfaD and the flanking DNA has been determined, and rfaD would appear to be the only gene homologous to known LPS core biosynthesis genes in this region. Comparison with the E. coli rfaD shows many similar structural features such as the ADP-binding βαβ fold at the N terminus, as well as a high degree of homology of both the nucleotide and amino-acid sequences. Based on homology, rfaD of V. cholerae may be transcribed using both σ 70-and σ 54-dependent promoters.

AB - The rfaD gene of Escherichia coli encodes ADP-L-glycero-D-mannoheptose-6-epimerase, an enzyme required for the biosynthesis of the lipopolysaccharide (LPS) precursor ADP-L-glycero-v-mannoheptose, associated with production of the core oligosaccharide. We have identified an rfaD homologue in Vibrio cholerae 01. This gene maps adjacent to the rfb region encoding O-antigen biosynthesis, but is transcribed divergently. The complete nucleotide sequence of rfaD and the flanking DNA has been determined, and rfaD would appear to be the only gene homologous to known LPS core biosynthesis genes in this region. Comparison with the E. coli rfaD shows many similar structural features such as the ADP-binding βαβ fold at the N terminus, as well as a high degree of homology of both the nucleotide and amino-acid sequences. Based on homology, rfaD of V. cholerae may be transcribed using both σ 70-and σ 54-dependent promoters.

KW - ADP-binding fold

KW - Lipopolysaccharide

KW - core oligosaccharide

KW - heptose biosynthesis

UR - https://www.scopus.com/pages/publications/0028986385

U2 - 10.1016/0378-1119(94)00923-G

DO - 10.1016/0378-1119(94)00923-G

M3 - Article

C2 - 7698669

AN - SCOPUS:0028986385

SN - 0378-1119

VL - 155

SP - 67

EP - 72

JO - Gene

JF - Gene

IS - 1

ER -

In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this