Interleukin 2 receptor regulation and IL-2 function in the human infant

H. Zola; J. Ridings; S. Elliott; S. Nobbs; H. Weedon; L. Wheatland; R. Haslam; D. Roberton; P. J. Macardle

doi:10.1016/S0198-8859(98)00069-X

Interleukin 2 receptor regulation and IL-2 function in the human infant

H. Zola, J. Ridings, S. Elliott, S. Nobbs, H. Weedon, L. Wheatland, R. Haslam, D. Roberton, P. J. Macardle

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

IL-2 receptor is expressed at low levels on adult blood lymphocytes, and at lower levels on cord blood cells. IL-2 receptor α and β chain expression increases gradually from 0-18 months of age. The level of soluble CD25 (IL-2 receptor α chain) has been reported to be elevated in cord blood. Quantitative RT-PCR showed that adult cells express 10 times as much CD25 mRNA as cord cells. Cord plasma showed only a marginal ability to strip CD25 from the membrane. To assess the functional consequences of low IL-2 receptor expression, cord and adult cells were activated in vitro. The response was stimulus-dependent, but cord cells upregulated CD25 readily. Cord and adult cells proliferated in an IL-2-dependent assay to a similar extent. Infants suffering acute infection showed marginally higher levels of membrane CD25 expression than infants without overt infection. Thus neonatal and infant lymphocytes express lower levels of IL-2 receptors than adult cells, reflecting lower mRNA concentrations at least for CD25; they are able to up- regulate receptors in response to in vitro stimulation and are able to respond in vitro to IL-2-dependent stimulation; however in vivo there may be a dampening down of the IL-2 system in infancy.

Original language	English
Pages (from-to)	615-624
Number of pages	10
Journal	Human Immunology
Volume	59
Issue number	10
DOIs	https://doi.org/10.1016/S0198-8859(98)00069-X
Publication status	Published or Issued - Oct 1998
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/S0198-8859(98)00069-X

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title = "Interleukin 2 receptor regulation and IL-2 function in the human infant",

abstract = "IL-2 receptor is expressed at low levels on adult blood lymphocytes, and at lower levels on cord blood cells. IL-2 receptor α and β chain expression increases gradually from 0-18 months of age. The level of soluble CD25 (IL-2 receptor α chain) has been reported to be elevated in cord blood. Quantitative RT-PCR showed that adult cells express 10 times as much CD25 mRNA as cord cells. Cord plasma showed only a marginal ability to strip CD25 from the membrane. To assess the functional consequences of low IL-2 receptor expression, cord and adult cells were activated in vitro. The response was stimulus-dependent, but cord cells upregulated CD25 readily. Cord and adult cells proliferated in an IL-2-dependent assay to a similar extent. Infants suffering acute infection showed marginally higher levels of membrane CD25 expression than infants without overt infection. Thus neonatal and infant lymphocytes express lower levels of IL-2 receptors than adult cells, reflecting lower mRNA concentrations at least for CD25; they are able to up- regulate receptors in response to in vitro stimulation and are able to respond in vitro to IL-2-dependent stimulation; however in vivo there may be a dampening down of the IL-2 system in infancy.",

author = "H. Zola and J. Ridings and S. Elliott and S. Nobbs and H. Weedon and L. Wheatland and R. Haslam and D. Roberton and Macardle, {P. J.}",

note = "Funding Information: This work was supported by a Project grant from the Australian National Health and Medical Research Council, and an equipment grant from the Variety Club, South Australia. We are grateful to Ms Sue Brandford, Institute of Medical and Veterinary Science (IMVS, Adelaide) and Ms Justine Elliott and Dr. Brant Bassam (Perkin Elmer Cetus, Melbourne) for help with the quantitative PCR, and to the IMVS for time on the ABI Prism 7700 sequence detector.",

year = "1998",

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T1 - Interleukin 2 receptor regulation and IL-2 function in the human infant

AU - Zola, H.

AU - Ridings, J.

AU - Elliott, S.

AU - Nobbs, S.

AU - Weedon, H.

AU - Wheatland, L.

AU - Haslam, R.

AU - Roberton, D.

AU - Macardle, P. J.

N1 - Funding Information: This work was supported by a Project grant from the Australian National Health and Medical Research Council, and an equipment grant from the Variety Club, South Australia. We are grateful to Ms Sue Brandford, Institute of Medical and Veterinary Science (IMVS, Adelaide) and Ms Justine Elliott and Dr. Brant Bassam (Perkin Elmer Cetus, Melbourne) for help with the quantitative PCR, and to the IMVS for time on the ABI Prism 7700 sequence detector.

PY - 1998/10

Y1 - 1998/10

N2 - IL-2 receptor is expressed at low levels on adult blood lymphocytes, and at lower levels on cord blood cells. IL-2 receptor α and β chain expression increases gradually from 0-18 months of age. The level of soluble CD25 (IL-2 receptor α chain) has been reported to be elevated in cord blood. Quantitative RT-PCR showed that adult cells express 10 times as much CD25 mRNA as cord cells. Cord plasma showed only a marginal ability to strip CD25 from the membrane. To assess the functional consequences of low IL-2 receptor expression, cord and adult cells were activated in vitro. The response was stimulus-dependent, but cord cells upregulated CD25 readily. Cord and adult cells proliferated in an IL-2-dependent assay to a similar extent. Infants suffering acute infection showed marginally higher levels of membrane CD25 expression than infants without overt infection. Thus neonatal and infant lymphocytes express lower levels of IL-2 receptors than adult cells, reflecting lower mRNA concentrations at least for CD25; they are able to up- regulate receptors in response to in vitro stimulation and are able to respond in vitro to IL-2-dependent stimulation; however in vivo there may be a dampening down of the IL-2 system in infancy.

AB - IL-2 receptor is expressed at low levels on adult blood lymphocytes, and at lower levels on cord blood cells. IL-2 receptor α and β chain expression increases gradually from 0-18 months of age. The level of soluble CD25 (IL-2 receptor α chain) has been reported to be elevated in cord blood. Quantitative RT-PCR showed that adult cells express 10 times as much CD25 mRNA as cord cells. Cord plasma showed only a marginal ability to strip CD25 from the membrane. To assess the functional consequences of low IL-2 receptor expression, cord and adult cells were activated in vitro. The response was stimulus-dependent, but cord cells upregulated CD25 readily. Cord and adult cells proliferated in an IL-2-dependent assay to a similar extent. Infants suffering acute infection showed marginally higher levels of membrane CD25 expression than infants without overt infection. Thus neonatal and infant lymphocytes express lower levels of IL-2 receptors than adult cells, reflecting lower mRNA concentrations at least for CD25; they are able to up- regulate receptors in response to in vitro stimulation and are able to respond in vitro to IL-2-dependent stimulation; however in vivo there may be a dampening down of the IL-2 system in infancy.

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Interleukin 2 receptor regulation and IL-2 function in the human infant

Abstract

ASJC Scopus subject areas

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