TY - JOUR
T1 - Liquid gastric emptying in the pig
T2 - Effect of concentration of inhaled isoflurane
AU - Anderson, Deirdre L.
AU - Bartholomeusz, F. Dylan
AU - Kirkwood, Ian D.
AU - Chatterton, Barry E.
AU - Summersides, Glenda
AU - Penglis, Stamatos
AU - Kuchel, Timothy
AU - Sansom, Lloyd
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - An animal model of gastric emptying may have use in the study of gastric physiology and pharmacoscintigraphy. The pig has anatomy and physiology similar to that of humans. Our aim was to develop a model of gastric emptying in the pig. It was not possible to perform this study in conscious pigs; therefore, an anesthetic model was developed. Methods: Fifteen studies were performed on 4 pigs (age, 2-6 mo; weight, 20-100 kg). After acclimatization and training, pigs were fasted overnight before the study. Pigs were anesthetized using inhaled isoflurane without the use of injected premedication agents. An orogastric tube was inserted for the administration of a liquid meal, which consisted of 99mTc-diethylenetriaminepentaacetic acid either in water (nonnutrient) or with dextrose (nutrient meal). The pig was laterally positioned to enable right lateral dynamic acquisition to be performed. Anesthesia was maintained at 2% ± 0.5% isoflurane in 4 studies and 0.8% ± 0.5% in 11 studies (4 nutrient, 7 nonnutrient). Results: With 2% ± 0.5% isoflurane, there was delayed gastric emptying with a mean 50% emptying time (±SEM) of 141 ± 14 min. With 0.8% ± 0.5% isoflurane, the liquid meal emptied in an exponential manner similar to that of humans, with mean 50% emptying times (±SEM) of 30 ± 7 min (nutrient) and 31 ± 4 min (nonnutrient). Conclusion: The results indicate that high-dose anesthesia inhibits gastric emptying, but with low-dose anesthesia a useful pig model of liquid gastric emptying can be developed.
AB - An animal model of gastric emptying may have use in the study of gastric physiology and pharmacoscintigraphy. The pig has anatomy and physiology similar to that of humans. Our aim was to develop a model of gastric emptying in the pig. It was not possible to perform this study in conscious pigs; therefore, an anesthetic model was developed. Methods: Fifteen studies were performed on 4 pigs (age, 2-6 mo; weight, 20-100 kg). After acclimatization and training, pigs were fasted overnight before the study. Pigs were anesthetized using inhaled isoflurane without the use of injected premedication agents. An orogastric tube was inserted for the administration of a liquid meal, which consisted of 99mTc-diethylenetriaminepentaacetic acid either in water (nonnutrient) or with dextrose (nutrient meal). The pig was laterally positioned to enable right lateral dynamic acquisition to be performed. Anesthesia was maintained at 2% ± 0.5% isoflurane in 4 studies and 0.8% ± 0.5% in 11 studies (4 nutrient, 7 nonnutrient). Results: With 2% ± 0.5% isoflurane, there was delayed gastric emptying with a mean 50% emptying time (±SEM) of 141 ± 14 min. With 0.8% ± 0.5% isoflurane, the liquid meal emptied in an exponential manner similar to that of humans, with mean 50% emptying times (±SEM) of 30 ± 7 min (nutrient) and 31 ± 4 min (nonnutrient). Conclusion: The results indicate that high-dose anesthesia inhibits gastric emptying, but with low-dose anesthesia a useful pig model of liquid gastric emptying can be developed.
KW - Anesthesia
KW - Gastric emptying
KW - Pig
KW - Scintigraphy
UR - http://www.scopus.com/inward/record.url?scp=0036301239&partnerID=8YFLogxK
M3 - Article
C2 - 12097470
AN - SCOPUS:0036301239
SN - 0161-5505
VL - 43
SP - 968
EP - 971
JO - Journal of Nuclear Medicine
JF - Journal of Nuclear Medicine
IS - 7
ER -