Local delivery of antimicrobial peptides using self-organized TiO 2 nanotube arrays for peri-implant infections

Menghan Ma, Mehdi Kazemzadeh-Narbat, Yu Hui, Shanshan Lu, Chuanfan Ding, David D.Y. Chen, Robert E.W. Hancock, Rizhi Wang

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128 Citations (Scopus)


Peri-implant infections have been reported as one of the major complications that lead to the failure of orthopedic implants. An ideal solution to the peri-implant infection is to locally deliver antimicrobial agents through the implant surface. The rising problem of infections caused by multiple antibiotic-resistant bacteria makes traditional antibiotics less desirable for the prevention of peri-implant infections. One of the promising alternatives is the family of antimicrobial peptides (AMPs). In this study, we report the local delivery of AMPs through the nanotubular structure processed on titanium surface. Self-organized and vertically oriented TiO 2 nanotubes, about 80 nm in diameter and 7 μm thick, were prepared by the anodization technique. HHC-36 (KRWWKWWRR), one of the most potent broad-spectrum AMPs, was loaded onto the TiO 2 nanotubes via a simple vacuum-assisted physical adsorption method. Antimicrobial activity testing against Gram-positive bacterium, Staphylococcus aureus, demonstrated that this AMP-loaded nanotubular surface could effectively kill the bacteria (Ëœ99.9% killing) and reduce the total bacterial number adhered to the surface after 4 h of culture. In vitro AMP elution from the nanotubes was investigated using liquid chromatography-mass spectrometry (LC-MS). The release profiles strongly depended on the crystallinity of the TiO 2 nanotubes. Anatase TiO 2 nanotubes released significantly higher amounts of AMP than amorphous nanotubes during the initial burst release stage. Both followed almost the same slow release profile from 4 h up to 7 days. Despite the differences in release kinetics, no significant difference was observed between these two groups in bactericidal efficiency.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number2
Publication statusPublished or Issued - Feb 2012
Externally publishedYes


  • TiO nanotubes
  • anodization
  • antimicrobial peptide
  • orthopaedic implants
  • peri-implant infection

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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