Abstract
The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.
Original language | English |
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Pages (from-to) | 319-323 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 532 |
Issue number | 3 |
DOIs | |
Publication status | Published or Issued - 18 Dec 2002 |
Externally published | Yes |
Keywords
- Nucleus
- Primary T cell
- Regulation
- eIF4E-binding protein 1
- eIF4E-binding protein 3
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology