Localisation and regulation of the eIF4E-binding protein 4E-BP3

Miranda Kleijn, Gert C. Scheper, Mary L. Wilson, Andrew R. Tee, Christopher G. Proud

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20 Citations (Scopus)


The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalFEBS Letters
Issue number3
Publication statusPublished or Issued - 18 Dec 2002
Externally publishedYes


  • Nucleus
  • Primary T cell
  • Regulation
  • eIF4E-binding protein 1
  • eIF4E-binding protein 3

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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