Localisation and regulation of the eIF4E-binding protein 4E-BP3

Miranda Kleijn; Gert C. Scheper; Mary L. Wilson; Andrew R. Tee; Christopher G. Proud

doi:10.1016/S0014-5793(02)03694-3

Localisation and regulation of the eIF4E-binding protein 4E-BP3

Miranda Kleijn, Gert C. Scheper, Mary L. Wilson, Andrew R. Tee, Christopher G. Proud

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.

Original language	English
Pages (from-to)	319-323
Number of pages	5
Journal	FEBS Letters
Volume	532
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(02)03694-3
Publication status	Published or Issued - 18 Dec 2002
Externally published	Yes

Keywords

Nucleus
Primary T cell
Regulation
eIF4E-binding protein 1
eIF4E-binding protein 3

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/S0014-5793(02)03694-3

Cite this

@article{7db1c4563f64466ba85271be6aa043e9,

title = "Localisation and regulation of the eIF4E-binding protein 4E-BP3",

abstract = "The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.",

keywords = "Nucleus, Primary T cell, Regulation, eIF4E-binding protein 1, eIF4E-binding protein 3",

author = "Miranda Kleijn and Scheper, {Gert C.} and Wilson, {Mary L.} and Tee, {Andrew R.} and Proud, {Christopher G.}",

note = "Funding Information: We would like to thank the Scottish National Blood Transfusion Service (Edinburgh, UK) for providing the buffy coats used for the isolation of T cells. This work was supported by a European Union TMR grant (ERBF MRXCT 980197) and by an MRC programme grant (G9901450).",

year = "2002",

month = dec,

day = "18",

doi = "10.1016/S0014-5793(02)03694-3",

language = "English",

volume = "532",

pages = "319--323",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "3",

}

TY - JOUR

T1 - Localisation and regulation of the eIF4E-binding protein 4E-BP3

AU - Kleijn, Miranda

AU - Scheper, Gert C.

AU - Wilson, Mary L.

AU - Tee, Andrew R.

AU - Proud, Christopher G.

N1 - Funding Information: We would like to thank the Scottish National Blood Transfusion Service (Edinburgh, UK) for providing the buffy coats used for the isolation of T cells. This work was supported by a European Union TMR grant (ERBF MRXCT 980197) and by an MRC programme grant (G9901450).

PY - 2002/12/18

Y1 - 2002/12/18

N2 - The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.

AB - The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.

KW - Nucleus

KW - Primary T cell

KW - Regulation

KW - eIF4E-binding protein 1

KW - eIF4E-binding protein 3

UR - http://www.scopus.com/inward/record.url?scp=0037132553&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(02)03694-3

DO - 10.1016/S0014-5793(02)03694-3

M3 - Article

C2 - 12482586

AN - SCOPUS:0037132553

SN - 0014-5793

VL - 532

SP - 319

EP - 323

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Localisation and regulation of the eIF4E-binding protein 4E-BP3

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this