MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

Richard Hummel; Corina Sie; David I. Watson; Tingting Wang; Alfiya Ansar; Michael Z. Michael; Mark Van Der Hoek; Joerg Haier; Damian J. Hussey

doi:10.3748/wjg.v20.i40.14904

MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

Richard Hummel, Corina Sie, David I. Watson, Tingting Wang, Alfiya Ansar, Michael Z. Michael, Mark Van Der Hoek, Joerg Haier, Damian J. Hussey

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

Original language	English
Pages (from-to)	14904-14912
Number of pages	9
Journal	World Journal of Gastroenterology
Volume	20
Issue number	40
DOIs	https://doi.org/10.3748/wjg.v20.i40.14904
Publication status	Published or Issued - 28 Oct 2014
Externally published	Yes

Keywords

Chemotherapy
Esophageal cancer
MicroRNA
Resistance
Target

ASJC Scopus subject areas

Gastroenterology

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10.3748/wjg.v20.i40.14904

Cite this

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title = "MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines",

abstract = "AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.",

keywords = "Chemotherapy, Esophageal cancer, MicroRNA, Resistance, Target",

author = "Richard Hummel and Corina Sie and Watson, {David I.} and Tingting Wang and Alfiya Ansar and Michael, {Michael Z.} and {Van Der Hoek}, Mark and Joerg Haier and Hussey, {Damian J.}",

year = "2014",

month = oct,

day = "28",

doi = "10.3748/wjg.v20.i40.14904",

language = "English",

volume = "20",

pages = "14904--14912",

journal = "World Journal of Gastroenterology",

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TY - JOUR

T1 - MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

AU - Hummel, Richard

AU - Sie, Corina

AU - Watson, David I.

AU - Wang, Tingting

AU - Ansar, Alfiya

AU - Michael, Michael Z.

AU - Van Der Hoek, Mark

AU - Haier, Joerg

AU - Hussey, Damian J.

PY - 2014/10/28

Y1 - 2014/10/28

N2 - AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

AB - AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

KW - Chemotherapy

KW - Esophageal cancer

KW - MicroRNA

KW - Resistance

KW - Target

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JF - World Journal of Gastroenterology

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MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

Abstract

Keywords

ASJC Scopus subject areas

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