MicroRNA signatures in chemotherapy resistant esophageal cancer cell lines

Richard Hummel, Corina Sie, David I. Watson, Tingting Wang, Alfiya Ansar, Michael Z. Michael, Mark Van Der Hoek, Joerg Haier, Damian J. Hussey

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

AIM: To investigate expression of microRNA (miRNA) and potential targets in chemotherapy resistant esophageal cancer cell lines. METHODS: An in-vitro model of acquired chemotherapy resistance in esophageal adeno- (EAC) and squamous cell carcinoma (ESCC) cells was used, and microRNA expression profiles for cisplatin or 5-fluorouracil (5-FU) resistant variants vs chemotherapy sensitive controls were compared using microarray and quantitative real-time polymerase chain reaction (PCR). The expression of chemotherapy-relevant genes potentially targeted by the dysregulated microRNAs in the chemotherapy resistant variants was also evaluated. RESULTS: Chemotherapy resistant sublines were found to have specific miRNA signatures, and these miRNA signatures were different for the cisplatin vs 5-FU resistant cells from the same tumor cell line, and also for EAC vs ESCC cells with resistance to the same specific chemotherapy agent. Amongst others, miR-27b-3p, miR-193b-3p, miR-192-5p, miR-378 a-3p, miR-125a-5p and miR-18a-3p were dysregulated, consistent with negative posttranscriptional control of KRAS, TYMS, ABCC3, CBL-B and ERBB2 expression via these miRNAs. CONCLUSION: The current study supports the hypothesis that microRNA expression has an impact on chemotherapy resistance in esophageal cancer.

Original languageEnglish
Pages (from-to)14904-14912
Number of pages9
JournalWorld Journal of Gastroenterology
Volume20
Issue number40
DOIs
Publication statusPublished or Issued - 28 Oct 2014
Externally publishedYes

Keywords

  • Chemotherapy
  • Esophageal cancer
  • MicroRNA
  • Resistance
  • Target

ASJC Scopus subject areas

  • Gastroenterology

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