TY - JOUR
T1 - Peptide arrays on cellulose support
T2 - SPOT synthesis, a time and cost efficient method for synthesis of large numbers of peptides in a parallel and addressable fashion
AU - Hilpert, Kai
AU - Winkler, Dirk F.H.
AU - Hancock, Robert E.W.
N1 - Funding Information:
ACKNOWLEDGMENTS We are grateful for financial assistance from the Applied Food and Materials Network and the Canadian Institutes of Health Research. R.E.W.H. was supported by a Canada Research Chair award. K.H. was supported by a fellowship from the Canadian Institutes of Health Research. We thank R.A. Klady for the critical proofreading of the manuscript.
PY - 2007/6
Y1 - 2007/6
N2 - Peptide synthesis on cellulose using SPOT technology allows the parallel synthesis of large numbers of addressable peptides in small amounts. In addition, the cost per peptide is less than 1% of peptides synthesized conventionally on resin. The SPOT method follows standard fluorenyl-methoxy-carbonyl chemistry on conventional cellulose sheets, and can utilize more than 600 different building blocks. The procedure involves three phases: preparation of the cellulose membrane, stepwise coupling of the amino acids and cleavage of the side-chain protection groups. If necessary, peptides can be cleaved from the membrane for assays performed using soluble peptides. These features make this method an excellent tool for screening large numbers of peptides for many different purposes. Potential applications range from simple binding assays, to more sophisticated enzyme assays and studies with living microbes or cells. The time required to complete the protocol depends on the number and length of the peptides. For example, 400 9-mer peptides can be synthesized within 6 days.
AB - Peptide synthesis on cellulose using SPOT technology allows the parallel synthesis of large numbers of addressable peptides in small amounts. In addition, the cost per peptide is less than 1% of peptides synthesized conventionally on resin. The SPOT method follows standard fluorenyl-methoxy-carbonyl chemistry on conventional cellulose sheets, and can utilize more than 600 different building blocks. The procedure involves three phases: preparation of the cellulose membrane, stepwise coupling of the amino acids and cleavage of the side-chain protection groups. If necessary, peptides can be cleaved from the membrane for assays performed using soluble peptides. These features make this method an excellent tool for screening large numbers of peptides for many different purposes. Potential applications range from simple binding assays, to more sophisticated enzyme assays and studies with living microbes or cells. The time required to complete the protocol depends on the number and length of the peptides. For example, 400 9-mer peptides can be synthesized within 6 days.
UR - http://www.scopus.com/inward/record.url?scp=34250721648&partnerID=8YFLogxK
U2 - 10.1038/nprot.2007.160
DO - 10.1038/nprot.2007.160
M3 - Article
C2 - 17545971
AN - SCOPUS:34250721648
SN - 1754-2189
VL - 2
SP - 1333
EP - 1349
JO - Nature Protocols
JF - Nature Protocols
IS - 6
ER -