Phosphorylation of elongation factor-2 from the lepidopteran insect, spodoptera frugiperda

Susan Oldfield, Christopher G. Proud

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6 Citations (Scopus)


In mammalian cells, protein synthesis can be regulated at the level of elongation by the phosphorylation of elongation factor 2 (eEF-2) by a highly specific Ca2+/calmodulin-dependent kinase. In this report, we show that eEF-2 from a cell line derived from the insect, Spodoptera frugiperda, is a substrate for mammalian eEF-2 kinase and that phosphorylation is Ca2+-dependent. Furthermore, two-dimensional peptide mapping shows that the kinase phosphorylates the same sites in Spodoptera eEF-2 as those phosphorylated in the rabbit protein. However, we were unable to detect an eEF-2 kinase in Spodoptera cells.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalFEBS Letters
Issue number1
Publication statusPublished or Issued - 19 Jul 1993
Externally publishedYes


  • Elongation factor 2
  • Phosphorylation
  • Protein synthesis
  • Spodoptera

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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