TY - JOUR
T1 - Physical and transcriptional mapping of DXS56-PGK1 1 mb region
T2 - Identification of three new transcripts
AU - Gecz, J.
AU - Vlllard, L.
AU - Lossl, A. M.
AU - Mlllasseau, P.
AU - Djaball, M.
AU - Fontes, M.
N1 - Funding Information:
We would like to thank A.P.Monaco for sending YAC clones through the ICRF Reference library System, to D. Le Paslier for providing the CEPH YACs and to J.Weissenbach for die access to T^-nrmg facilities. TUs work was 1144x11ted by grants from MRT (ACC 'Genome humam1), EEC fHuman genome analysis' ptuginu) and Association Francaise Centre les Myopamies, J.Gecz was supported by a fellowship of 'Institut Electrics* Saute".
PY - 1993/9
Y1 - 1993/9
N2 - Several new techniques for insolation expressed sequences have been recently described considerably speeding up the Identification of unknown genes. Here we present a transcriptional map of the 1 Mb DXSS6-PGK1 region in Xq13.3. Rare cutter restriction site mapping, direct cDNA selection on membrane discs and probing of Northern blots with total YAC DNA, were the methods explored In order to achieve this goal. In addition to three known genes from this region which have been recloned, two new cDNA clones correnponding to two new genes were Isolated, mapped and characterized. Moreover one more transcript, highly expressed in placenta, has been detected in the region with a total YAC as a probe. In summary there are at least six genes known to reside in the DXS56-PGK1 region. As several human disease gene locl (i.e. SCID, CMTX1, WWS, MRX, XDP, ASB) were tightly linked to the markers from the region (PGK, CA repeats), the three new transcripts may be considered as their potential candidate genes.
AB - Several new techniques for insolation expressed sequences have been recently described considerably speeding up the Identification of unknown genes. Here we present a transcriptional map of the 1 Mb DXSS6-PGK1 region in Xq13.3. Rare cutter restriction site mapping, direct cDNA selection on membrane discs and probing of Northern blots with total YAC DNA, were the methods explored In order to achieve this goal. In addition to three known genes from this region which have been recloned, two new cDNA clones correnponding to two new genes were Isolated, mapped and characterized. Moreover one more transcript, highly expressed in placenta, has been detected in the region with a total YAC as a probe. In summary there are at least six genes known to reside in the DXS56-PGK1 region. As several human disease gene locl (i.e. SCID, CMTX1, WWS, MRX, XDP, ASB) were tightly linked to the markers from the region (PGK, CA repeats), the three new transcripts may be considered as their potential candidate genes.
UR - http://www.scopus.com/inward/record.url?scp=0027184220&partnerID=8YFLogxK
U2 - 10.1093/hmg/2.9.1389
DO - 10.1093/hmg/2.9.1389
M3 - Article
C2 - 8242062
AN - SCOPUS:0027184220
SN - 0964-6906
VL - 2
SP - 1389
EP - 1396
JO - Human molecular genetics
JF - Human molecular genetics
IS - 9
ER -