PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Ian C. Nicholson; Christos Mavrangelos; Daniel R G Bird; Suzanne Bresatz-Atkins; Nicola G. Eastaff-Leung; Randall H. Grose; Batjargal Gundsambuu; Danika Hill; Debbrah J. Millard; Timothy J. Sadlon; Sarah To; Heddy Zola; Simon C. Barry; Doreen Krumbiegel

doi:10.1016/j.cellimm.2012.04.002

PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Ian C. Nicholson, Christos Mavrangelos, Daniel R G Bird, Suzanne Bresatz-Atkins, Nicola G. Eastaff-Leung, Randall H. Grose, Batjargal Gundsambuu, Danika Hill, Debbrah J. Millard, Timothy J. Sadlon, Sarah To, Heddy Zola, Simon C. Barry, Doreen Krumbiegel

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Abstract

The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

Original language	English
Pages (from-to)	12-18
Number of pages	7
Journal	Cellular Immunology
Volume	275
Issue number	1-2
DOIs	https://doi.org/10.1016/j.cellimm.2012.04.002
Publication status	Published or Issued - Jan 2012

Keywords

Lymphocyte migration
Memory Treg
Peptidase inhibitor 16
Regulatory T cells

ASJC Scopus subject areas

Immunology

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10.1016/j.cellimm.2012.04.002

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Nicholson, I. C., Mavrangelos, C., Bird, D. R. G., Bresatz-Atkins, S., Eastaff-Leung, N. G., Grose, R. H., Gundsambuu, B., Hill, D., Millard, D. J., Sadlon, T. J., To, S., Zola, H., Barry, S. C., & Krumbiegel, D. (2012). PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20. Cellular Immunology, 275(1-2), 12-18. https://doi.org/10.1016/j.cellimm.2012.04.002

@article{b80601a23e7546c7897dccc1791fb73a,

title = "PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20",

abstract = "The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.",

keywords = "Lymphocyte migration, Memory Treg, Peptidase inhibitor 16, Regulatory T cells",

author = "Nicholson, {Ian C.} and Christos Mavrangelos and Bird, {Daniel R G} and Suzanne Bresatz-Atkins and Eastaff-Leung, {Nicola G.} and Grose, {Randall H.} and Batjargal Gundsambuu and Danika Hill and Millard, {Debbrah J.} and Sadlon, {Timothy J.} and Sarah To and Heddy Zola and Barry, {Simon C.} and Doreen Krumbiegel",

note = "Funding Information: This work was funded by the Co-operative Research Centre for Biomarker Translation. ",

year = "2012",

month = jan,

doi = "10.1016/j.cellimm.2012.04.002",

language = "English",

volume = "275",

pages = "12--18",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1-2",

}

Nicholson, IC, Mavrangelos, C, Bird, DRG, Bresatz-Atkins, S, Eastaff-Leung, NG, Grose, RH, Gundsambuu, B, Hill, D, Millard, DJ, Sadlon, TJ, To, S, Zola, H, Barry, SC & Krumbiegel, D 2012, 'PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20', Cellular Immunology, vol. 275, no. 1-2, pp. 12-18. https://doi.org/10.1016/j.cellimm.2012.04.002

TY - JOUR

T1 - PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

AU - Nicholson, Ian C.

AU - Mavrangelos, Christos

AU - Bird, Daniel R G

AU - Bresatz-Atkins, Suzanne

AU - Eastaff-Leung, Nicola G.

AU - Grose, Randall H.

AU - Gundsambuu, Batjargal

AU - Hill, Danika

AU - Millard, Debbrah J.

AU - Sadlon, Timothy J.

AU - To, Sarah

AU - Zola, Heddy

AU - Barry, Simon C.

AU - Krumbiegel, Doreen

N1 - Funding Information: This work was funded by the Co-operative Research Centre for Biomarker Translation.

PY - 2012/1

Y1 - 2012/1

N2 - The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

AB - The peptidase inhibitor PI16 was shown previously by microarray analysis to be over-expressed by CD4-positive/CD25-positive Treg compared with CD4-positive/CD25-negative Th cells. Using a monoclonal antibody to the human PI16 protein, we found that PI16-positive Treg have a memory (CD45RO-positive) phenotype and express higher levels of FOXP3 than PI16-negative Treg. PI16-positive Treg are functional in suppressor assays . in vitro with potency similar to PI16-negative Treg. Further phenotyping of the PI16-positive Treg revealed that the chemokine receptors CCR4 and CCR6 are expressed by more of the PI16-positive/CD45RO-positive Treg compared with PI16-negative/CD45RO-positive Treg or Th cells. PI16-positive Treg showed enhanced . in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation. We conclude that PI16 identifies a novel distinct subset of functional memory Treg which can migrate to sites of inflammation and regulate the pro-inflammatory response at those sites.

KW - Lymphocyte migration

KW - Memory Treg

KW - Peptidase inhibitor 16

KW - Regulatory T cells

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U2 - 10.1016/j.cellimm.2012.04.002

DO - 10.1016/j.cellimm.2012.04.002

M3 - Article

C2 - 22533972

AN - SCOPUS:84860551956

SN - 0008-8749

VL - 275

SP - 12

EP - 18

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1-2

ER -

PI16 is expressed by a subset of human memory Treg with enhanced migration to CCL17 and CCL20

Abstract

Keywords

ASJC Scopus subject areas

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