Platelet rich plasma and dentine effect on sheep dental pulp cells regeneration/revitalization ability (in vitro)

Background: Platelet rich plasma (PRP) has been proposed as a scaffold for pulp regeneration/revitalization instead of a blood clot. The aim of the following in vitro study was to evaluate the effect of PRP scaffold on proliferation, migration and differentiation of cultured ovine (sheep) dental pulp cells (ODPC) in the presence of dentine. Methods: PRP was prepared by centrifuging blood at 140 g for 12 min. ODPC were cultured on PRP or platelet poor plasma (PPP) scaffolds with and without dentine discs. Cell proliferation, migration and differentiation rates were assessed. Results: ODPC cultured on PRP scaffold showed significantly greater proliferation rates, migration and mineralization compared with cells on PPP or without a scaffold. Dentine discs reduced the proliferation and mineralization potential of the cells. Conclusions: A PRP scaffold has a positive effect on the proliferation, migration and differentiation of ODPC; however, dentine discs have an adverse effect on the activity of ODPC.