PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats

Achini K. Vidanapathirana; Leslie C. Thompson; Erin E. Mann; Jillian T. Odom; Nathan A. Holland; Susan J. Sumner; Li Han; Anita H. Lewin; Timothy R. Fennell; Jared M. Brown; Christopher J. Wingard

doi:10.1016/j.reprotox.2014.07.074

PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats

Achini K. Vidanapathirana, Leslie C. Thompson, Erin E. Mann, Jillian T. Odom, Nathan A. Holland, Susan J. Sumner, Li Han, Anita H. Lewin, Timothy R. Fennell, Jared M. Brown, Christopher J. Wingard

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Pregnancy is a unique physiological state, in which C60 fullerene is reported to be distributed in both maternal and fetal tissues. Tissue distribution of C60 differs between pregnant and non-pregnant states, presumably due to functional changes in vasculature during pregnancy. We hypothesized that polyvinylpyrrolidone (PVP) formulated C60 (C60/PVP) increases vascular tissue contractility during pregnancy by increasing Rho-kinase activity. C60/PVP was administered intravenously to pregnant and non-pregnant female Sprague Dawley rats. Vascular responses were assessed using wire myography 24. h post-exposure. Increased stress generation was observed in uterine artery, thoracic aorta and umbilical vein. Rho-Rho-kinase mediated force maintenance was increased in arterial segments from C60/PVP exposed pregnant rats when compared to PVP exposed rats. Our findings suggest that intravenous exposure to C60/PVP during pregnancy increases vascular tissue contractility of the uterine artery through elements of Rho-Rho-kinase signaling during late stages of pregnancy.

Original language	English
Pages (from-to)	86-100
Number of pages	15
Journal	Reproductive Toxicology
Volume	49
DOIs	https://doi.org/10.1016/j.reprotox.2014.07.074
Publication status	Published or Issued - Nov 2014
Externally published	Yes

Keywords

Nanotoxicology
Polyvinylpyrrolidone
Pregnancy
Rho-kinase pathway
Umbilical vein
Uterine artery
Vascular tissue contractility

ASJC Scopus subject areas

Toxicology

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10.1016/j.reprotox.2014.07.074

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Vidanapathirana, A. K., Thompson, L. C., Mann, E. E., Odom, J. T., Holland, N. A., Sumner, S. J., Han, L., Lewin, A. H., Fennell, T. R., Brown, J. M., & Wingard, C. J. (2014). PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats. Reproductive Toxicology, 49, 86-100. https://doi.org/10.1016/j.reprotox.2014.07.074

@article{cf718571c00b4bb4aaed9288d23e8baf,

title = "PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats",

abstract = "Pregnancy is a unique physiological state, in which C60 fullerene is reported to be distributed in both maternal and fetal tissues. Tissue distribution of C60 differs between pregnant and non-pregnant states, presumably due to functional changes in vasculature during pregnancy. We hypothesized that polyvinylpyrrolidone (PVP) formulated C60 (C60/PVP) increases vascular tissue contractility during pregnancy by increasing Rho-kinase activity. C60/PVP was administered intravenously to pregnant and non-pregnant female Sprague Dawley rats. Vascular responses were assessed using wire myography 24. h post-exposure. Increased stress generation was observed in uterine artery, thoracic aorta and umbilical vein. Rho-Rho-kinase mediated force maintenance was increased in arterial segments from C60/PVP exposed pregnant rats when compared to PVP exposed rats. Our findings suggest that intravenous exposure to C60/PVP during pregnancy increases vascular tissue contractility of the uterine artery through elements of Rho-Rho-kinase signaling during late stages of pregnancy.",

keywords = "Nanotoxicology, Polyvinylpyrrolidone, Pregnancy, Rho-kinase pathway, Umbilical vein, Uterine artery, Vascular tissue contractility",

author = "Vidanapathirana, {Achini K.} and Thompson, {Leslie C.} and Mann, {Erin E.} and Odom, {Jillian T.} and Holland, {Nathan A.} and Sumner, {Susan J.} and Li Han and Lewin, {Anita H.} and Fennell, {Timothy R.} and Brown, {Jared M.} and Wingard, {Christopher J.}",

note = "Funding Information: We would like to thank Catherine O'Sullivan who prepared all the vials of C60/PVP and PVP vehicle pellets, to Dr. Jonathan Shannahan for assistance in cytokine analysis and to Alyssa DaVolio for the assistance with the PCR. We are very grateful to Dr. Robert M. Lust and Shaun Reece for their guidance with cardiac ultrasonography. This project was funded by the National Institute of Environmental Health Sciences grant U19 ES019525 and an AHA Mid-Atlantic Affiliate Pre-doctoral Fellowship (13PRE16490007) to AKV. ",

year = "2014",

month = nov,

doi = "10.1016/j.reprotox.2014.07.074",

language = "English",

volume = "49",

pages = "86--100",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats

AU - Vidanapathirana, Achini K.

AU - Thompson, Leslie C.

AU - Mann, Erin E.

AU - Odom, Jillian T.

AU - Holland, Nathan A.

AU - Sumner, Susan J.

AU - Han, Li

AU - Lewin, Anita H.

AU - Fennell, Timothy R.

AU - Brown, Jared M.

AU - Wingard, Christopher J.

N1 - Funding Information: We would like to thank Catherine O'Sullivan who prepared all the vials of C60/PVP and PVP vehicle pellets, to Dr. Jonathan Shannahan for assistance in cytokine analysis and to Alyssa DaVolio for the assistance with the PCR. We are very grateful to Dr. Robert M. Lust and Shaun Reece for their guidance with cardiac ultrasonography. This project was funded by the National Institute of Environmental Health Sciences grant U19 ES019525 and an AHA Mid-Atlantic Affiliate Pre-doctoral Fellowship (13PRE16490007) to AKV.

PY - 2014/11

Y1 - 2014/11

N2 - Pregnancy is a unique physiological state, in which C60 fullerene is reported to be distributed in both maternal and fetal tissues. Tissue distribution of C60 differs between pregnant and non-pregnant states, presumably due to functional changes in vasculature during pregnancy. We hypothesized that polyvinylpyrrolidone (PVP) formulated C60 (C60/PVP) increases vascular tissue contractility during pregnancy by increasing Rho-kinase activity. C60/PVP was administered intravenously to pregnant and non-pregnant female Sprague Dawley rats. Vascular responses were assessed using wire myography 24. h post-exposure. Increased stress generation was observed in uterine artery, thoracic aorta and umbilical vein. Rho-Rho-kinase mediated force maintenance was increased in arterial segments from C60/PVP exposed pregnant rats when compared to PVP exposed rats. Our findings suggest that intravenous exposure to C60/PVP during pregnancy increases vascular tissue contractility of the uterine artery through elements of Rho-Rho-kinase signaling during late stages of pregnancy.

AB - Pregnancy is a unique physiological state, in which C60 fullerene is reported to be distributed in both maternal and fetal tissues. Tissue distribution of C60 differs between pregnant and non-pregnant states, presumably due to functional changes in vasculature during pregnancy. We hypothesized that polyvinylpyrrolidone (PVP) formulated C60 (C60/PVP) increases vascular tissue contractility during pregnancy by increasing Rho-kinase activity. C60/PVP was administered intravenously to pregnant and non-pregnant female Sprague Dawley rats. Vascular responses were assessed using wire myography 24. h post-exposure. Increased stress generation was observed in uterine artery, thoracic aorta and umbilical vein. Rho-Rho-kinase mediated force maintenance was increased in arterial segments from C60/PVP exposed pregnant rats when compared to PVP exposed rats. Our findings suggest that intravenous exposure to C60/PVP during pregnancy increases vascular tissue contractility of the uterine artery through elements of Rho-Rho-kinase signaling during late stages of pregnancy.

KW - Nanotoxicology

KW - Polyvinylpyrrolidone

KW - Pregnancy

KW - Rho-kinase pathway

KW - Umbilical vein

KW - Uterine artery

KW - Vascular tissue contractility

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U2 - 10.1016/j.reprotox.2014.07.074

DO - 10.1016/j.reprotox.2014.07.074

M3 - Article

C2 - 25088243

AN - SCOPUS:84907303241

SN - 0890-6238

VL - 49

SP - 86

EP - 100

JO - Reproductive Toxicology

JF - Reproductive Toxicology

ER -

PVP formulated fullerene (C60) increases Rho-kinase dependent vascular tissue contractility in pregnant Sprague Dawley rats

Abstract

Keywords

ASJC Scopus subject areas

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