Rapamycin enhances eIF4E phosphorylation by activating MAP kinase-interacting kinase 2a (Mnk2a)

Rebecca L. Stead, Christopher G. Proud

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Eukaryotic initiation factor eIF4E and its phosphorylation play key roles in cell transformation and tumorigenesis. eIF4E is phosphorylated by the Mnks (MAP (mitogen-activated protein) kinase-interacting kinases). Rapamycin increases eIF4E phosphorylation in cancer cells, potentially limiting their anti-cancer effects. Here we show that the rapamycin-induced increase in eIF4E phosphorylation reflects increased activity of Mnk2 but not Mnk1. This activation requires a novel phosphorylation site in Mnk2a, Ser437. Our findings have potentially important implications for the use of rapamycin and its analogues in cancer therapy, suggesting that inhibitors of mTOR and Mnk (or Mnk2) may be more efficacious than rapalogs alone.

Original languageEnglish
Pages (from-to)2623-2628
Number of pages6
JournalFEBS Letters
Volume587
Issue number16
DOIs
Publication statusPublished or Issued - 19 Aug 2013
Externally publishedYes

Keywords

  • Mnk
  • Rapamycin
  • eIF4E
  • mTORC1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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