Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation

Christopher G. Proud; Michael J. Clemens; Virginia M. Pain

doi:10.1016/0014-5793(82)80810-7

Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation

Christopher G. Proud, Michael J. Clemens, Virginia M. Pain

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNA_f · GTP · eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.

Original language	English
Pages (from-to)	214-220
Number of pages	7
Journal	FEBS Letters
Volume	148
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(82)80810-7
Publication status	Published or Issued - 8 Nov 1982
Externally published	Yes

Keywords

Eukaryotic initiation factor-2
Protein synthesis
Reticulocyte lysate

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(82)80810-7

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title = "Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation",

abstract = "Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNAf · GTP · eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.",

keywords = "Eukaryotic initiation factor-2, Protein synthesis, Reticulocyte lysate",

author = "Proud, {Christopher G.} and Clemens, {Michael J.} and Pain, {Virginia M.}",

note = "Funding Information: We are very gratefult o Dr T. Hunt (University of Cambridge) for HCR, to Dr R. Panniers (Universityo f RochesterC ancer Center, NY) for guanine nucleotide exchange factor and to Vivienne Tilleray (St. George{\textquoteright}s Hospital Medical School) for preparingr at liver eIF-2. This work wass upportedb y a grantt o V.M.P. from the Medical ResearchC ouncil. M.J.C. is the recipiento f a Cancer ResearchC ampaignC areer Development Award.",

year = "1982",

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doi = "10.1016/0014-5793(82)80810-7",

language = "English",

volume = "148",

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journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

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T1 - Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation

AU - Proud, Christopher G.

AU - Clemens, Michael J.

AU - Pain, Virginia M.

N1 - Funding Information: We are very gratefult o Dr T. Hunt (University of Cambridge) for HCR, to Dr R. Panniers (Universityo f RochesterC ancer Center, NY) for guanine nucleotide exchange factor and to Vivienne Tilleray (St. George’s Hospital Medical School) for preparingr at liver eIF-2. This work wass upportedb y a grantt o V.M.P. from the Medical ResearchC ouncil. M.J.C. is the recipiento f a Cancer ResearchC ampaignC areer Development Award.

PY - 1982/11/8

Y1 - 1982/11/8

N2 - Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNAf · GTP · eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.

AB - Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNAf · GTP · eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.

KW - Eukaryotic initiation factor-2

KW - Protein synthesis

KW - Reticulocyte lysate

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DO - 10.1016/0014-5793(82)80810-7

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C2 - 6924897

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SN - 0014-5793

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

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Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation

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Keywords

ASJC Scopus subject areas

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