Regulation of binding of initiator tRNA to eukaryotic initiation factor eIF-2. Effects of the haem-controlled repressor on the kinetics of ternary complex formation

Christopher G. Proud, Michael J. Clemens, Virginia M. Pain

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


Ternary complex formation was studied in reticulocyte lysate supernatants and using rat liver eukaryotic initiation factor-2 (eIF-2) preparations. Haem-deficiency reduced the rate of formation of ternary (Met-tRNAf · GTP · eIF-2) complexes by the eIF-2 in reticulocyte supernatants, the reduction being more marked when complex formation was assayed in the absence of GTP-regenerating capacity. Pretreatment with the haem-controlled repressor (HCR) reduced the rate of ternary complex formation by crude (liver) eIF-2. In contrast, complex formation by an almost homogeneous eIF-2 preparation was unaffected by HCR: sensitivity to HCR was however restored by a factor which catalyses exchange of guanine nucleotides bound to eIF-2.

Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalFEBS Letters
Issue number2
Publication statusPublished or Issued - 8 Nov 1982
Externally publishedYes


  • Eukaryotic initiation factor-2
  • Protein synthesis
  • Reticulocyte lysate

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this