Regulation of polypeptide-chain initiation in rat skeletal muscle Starvation does not alter the activity or phosphorylation state of initiation factor eIF-2

Sarah Cox; Nicholas T. Redpath; Christopher G. Proud

doi:10.1016/0014-5793(88)80946-3

Regulation of polypeptide-chain initiation in rat skeletal muscle Starvation does not alter the activity or phosphorylation state of initiation factor eIF-2

Sarah Cox, Nicholas T. Redpath, Christopher G. Proud

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

In rats, 48-h starvation causes a decrease in the rate of protein synthesis in skeletal (e.g. gastrocnemius) muscle, due largely to impairment of peptide-chain initiation. In other cell types inhibition of initiation is associated with decreased activity and recycling of initiation factor eIF-2, and increased phosphorylation of its α-subunit. However, 48-h starvation has no effect on the activity or recycling of eIF-2 measured in extracts of gastrocnemius muscle, or on the level of α-subunit phosphorylation. The effects of starvation on peptide-chain initiation in skeletal muscle must therefore involve alterations in other components of the translational machinery.

Original language	English
Pages (from-to)	333-338
Number of pages	6
Journal	FEBS Letters
Volume	239
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(88)80946-3
Publication status	Published or Issued - 7 Nov 1988
Externally published	Yes

Keywords

Initiation
Initiation factor-2 (eIF-2)
Protein phosphorylation
Protein synthesis
Skeletal muscle

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(88)80946-3

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title = "Regulation of polypeptide-chain initiation in rat skeletal muscle Starvation does not alter the activity or phosphorylation state of initiation factor eIF-2",

abstract = "In rats, 48-h starvation causes a decrease in the rate of protein synthesis in skeletal (e.g. gastrocnemius) muscle, due largely to impairment of peptide-chain initiation. In other cell types inhibition of initiation is associated with decreased activity and recycling of initiation factor eIF-2, and increased phosphorylation of its α-subunit. However, 48-h starvation has no effect on the activity or recycling of eIF-2 measured in extracts of gastrocnemius muscle, or on the level of α-subunit phosphorylation. The effects of starvation on peptide-chain initiation in skeletal muscle must therefore involve alterations in other components of the translational machinery.",

keywords = "Initiation, Initiation factor-2 (eIF-2), Protein phosphorylation, Protein synthesis, Skeletal muscle",

author = "Sarah Cox and Redpath, {Nicholas T.} and Proud, {Christopher G.}",

note = "Funding Information: Acknowledgements: This work was supported in part by a Project Grant (to C.G.P.) from the Medical Research Council. S.C. and N.T.R. received Research Studentships, respectively, from the Science and Engineering Research Council and the Medical Research Council. We are grateful to David Colthurst who prepared the purified eIF-2 and the HCR used in this work, and to Drs Jenny Pain and Ian Jeffrey (Sussex University) for valuable advice and discussions. We are also.greatly indebted to Drs Ed Henshaw and Kathleen Scorsone (University of Rochester, New York) for providing the monoclonal antibody to eIF-2Lu used in this work.",

year = "1988",

month = nov,

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doi = "10.1016/0014-5793(88)80946-3",

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journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

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T1 - Regulation of polypeptide-chain initiation in rat skeletal muscle Starvation does not alter the activity or phosphorylation state of initiation factor eIF-2

AU - Cox, Sarah

AU - Redpath, Nicholas T.

AU - Proud, Christopher G.

N1 - Funding Information: Acknowledgements: This work was supported in part by a Project Grant (to C.G.P.) from the Medical Research Council. S.C. and N.T.R. received Research Studentships, respectively, from the Science and Engineering Research Council and the Medical Research Council. We are grateful to David Colthurst who prepared the purified eIF-2 and the HCR used in this work, and to Drs Jenny Pain and Ian Jeffrey (Sussex University) for valuable advice and discussions. We are also.greatly indebted to Drs Ed Henshaw and Kathleen Scorsone (University of Rochester, New York) for providing the monoclonal antibody to eIF-2Lu used in this work.

PY - 1988/11/7

Y1 - 1988/11/7

N2 - In rats, 48-h starvation causes a decrease in the rate of protein synthesis in skeletal (e.g. gastrocnemius) muscle, due largely to impairment of peptide-chain initiation. In other cell types inhibition of initiation is associated with decreased activity and recycling of initiation factor eIF-2, and increased phosphorylation of its α-subunit. However, 48-h starvation has no effect on the activity or recycling of eIF-2 measured in extracts of gastrocnemius muscle, or on the level of α-subunit phosphorylation. The effects of starvation on peptide-chain initiation in skeletal muscle must therefore involve alterations in other components of the translational machinery.

AB - In rats, 48-h starvation causes a decrease in the rate of protein synthesis in skeletal (e.g. gastrocnemius) muscle, due largely to impairment of peptide-chain initiation. In other cell types inhibition of initiation is associated with decreased activity and recycling of initiation factor eIF-2, and increased phosphorylation of its α-subunit. However, 48-h starvation has no effect on the activity or recycling of eIF-2 measured in extracts of gastrocnemius muscle, or on the level of α-subunit phosphorylation. The effects of starvation on peptide-chain initiation in skeletal muscle must therefore involve alterations in other components of the translational machinery.

KW - Initiation

KW - Initiation factor-2 (eIF-2)

KW - Protein phosphorylation

KW - Protein synthesis

KW - Skeletal muscle

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DO - 10.1016/0014-5793(88)80946-3

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AN - SCOPUS:0023685392

SN - 0014-5793

VL - 239

SP - 333

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Regulation of polypeptide-chain initiation in rat skeletal muscle Starvation does not alter the activity or phosphorylation state of initiation factor eIF-2

Abstract

Keywords

ASJC Scopus subject areas

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