TY - JOUR
T1 - RFLP-defined HLA-DP polymorphism in four ethnic groups
AU - Easteal, Simon
AU - Voorhoeve, Anne H.
AU - Zimmet, Paul
AU - Serjeantson, Susan W.
N1 - Funding Information:
Research Centre, Madras and R. L. Kirk, Australian National University, for providing the South Indian blood samples; B. R. Hawkins, University of Hong Kong, for providing the Chinese samples; and the Blood Transfusion Service, Canberra, for white Australian samples. D. Larhammar and A.-K. Jonsson, University of Uppsala, kindly provided the DNA probes. This work was supported in part by National Institutes of Health Grant AM 25446, and Juvenile Diabetes Foundation International Grant 18897~
PY - 1989/7
Y1 - 1989/7
N2 - Patterns of BglII, MspI, and TaqI DPα and DPß hybridizing restriction fragment length polymorphisms are compared with primed lymphocyte typing-determined specificities in 28 10th International Histocompatibility Workshop core cell lines. Correlation of specific RFLP patterns with most recognized DP types is confirmed, although the RFLPs do not distinguish DPw3 from DPw6. Analysis of DP-region nucleotide sequence data shows that the observed RFLPs are those expected to hybridize to the DPα and ß probes and are thus not due to cross-hybridization to other genes. The distribution of RFLPs in Chinese, Micronesian, South Indian, and white Australian populations is described. The most frequent DP specificity in Chinese is DPw5. In the other populations DPw4 is the most common specificity, although DPw6 is also relatively common in Micronesians. Four new DPα RFLP patterns and three new DPß patterns are described. There are also numerous unusual combinations of DPα and DPß alleles particularly in the South Indian population.
AB - Patterns of BglII, MspI, and TaqI DPα and DPß hybridizing restriction fragment length polymorphisms are compared with primed lymphocyte typing-determined specificities in 28 10th International Histocompatibility Workshop core cell lines. Correlation of specific RFLP patterns with most recognized DP types is confirmed, although the RFLPs do not distinguish DPw3 from DPw6. Analysis of DP-region nucleotide sequence data shows that the observed RFLPs are those expected to hybridize to the DPα and ß probes and are thus not due to cross-hybridization to other genes. The distribution of RFLPs in Chinese, Micronesian, South Indian, and white Australian populations is described. The most frequent DP specificity in Chinese is DPw5. In the other populations DPw4 is the most common specificity, although DPw6 is also relatively common in Micronesians. Four new DPα RFLP patterns and three new DPß patterns are described. There are also numerous unusual combinations of DPα and DPß alleles particularly in the South Indian population.
UR - http://www.scopus.com/inward/record.url?scp=0024396165&partnerID=8YFLogxK
U2 - 10.1016/0198-8859(89)90080-3
DO - 10.1016/0198-8859(89)90080-3
M3 - Article
C2 - 2570050
AN - SCOPUS:0024396165
SN - 0198-8859
VL - 25
SP - 169
EP - 179
JO - Human Immunology
JF - Human Immunology
IS - 3
ER -